Home / IL-10 / Porcine IL-10 Antibody

Porcine IL-10 Antibody

Catalog #: MAB693
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB693
MAB693-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-10 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL-10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL‑22 is observed and no cross-reactivity with recombinant IL-10 from cotton rat, canine, feline, human, mouse, or rat is observed.
Source
Monoclonal Mouse IgG2A Clone # 148806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant porcine IL-10
Ser19-Asn175
Accession # Q29055
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Porcine IL‑10 (Catalog # 693-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1+ (CD5+) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identitywith mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References
  1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Porcine IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
    Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
    MethodsX, 2019-05-17;6(0):1218-1227.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  2. In vitro effect of vitamin E on lectin-stimulated porcine peripheral blood mononuclear cells.
    Authors: Hernandez J, Soto-Canevett E, Pinelli-Saavedra A, Resendiz M, Moya-Camarena SY, Klasing KC
    Vet. Immunol. Immunopathol., 2009-03-14;131(1):9-16.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Porcine IL-10 Antibody

There are currently no reviews for this product. Be the first to review Porcine IL-10 Antibody and earn rewards!

Have you used Porcine IL-10 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review