Home / IL-12/IL-23 p40 / Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

Catalog #: BAM9122
2 Citations Datasheet / COA / SDS
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BAM9122
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Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL‑12/IL‑23 p40 Biotinylated Antibody Summary

Species Reactivity
Porcine
Specificity
Detects the p40 subunit of porcine IL-12 and IL-23. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human (rh) IL‑12 and rhIL‑23 and no cross-reactivity with rmIL-12 or rmIL-23.
Source
Monoclonal Mouse IgG2B Clone # 116211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Porcine IL-12 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Porcine IL‑12/IL‑23 p40 Antibody (Catalog # MAB9121)

Standard: Recombinant Porcine IL-12 Protein (Catalog # 912-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.

Long Name
Interleukin 12/Interleukin 23 p40
Entrez Gene IDs
3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)
Alternate Names
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

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Citations for Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
    Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
    Infect. Immun., 2017-03-23;85(4):.
    Species: Porcine
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome
    Authors: Andrea Ladinig, Joan K Lunney, Carlos J H Souza, Carolyn Ashley, Graham Plastow, John C S Harding
    Veterinary Research

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