Porcine IL-12/IL-35 p35 Antibody

Catalog #: MAB1171
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1171
MAB1171-SP

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-12/IL-35 p35 Antibody Summary

Species Reactivity

Porcine

Specificity

Detects the heterodimeric form of porcine IL‑12 (p35/p40) in direct ELISAs. Western blotting data indicates recognition of the IL-12/IL-35 p35 subunit. In direct ELISAs and Western blots, 20‑100% cross-reactivity with the disulfide‑linked heterodomeric forms of recombinant mouse IL‑12 and recombinant human IL-12 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 116219

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p35/p40 heterodimer
p40 Ile23-Asn324, p35 Arg26-Ser222
Accession # Q28938, Q29053

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Porcine IL-12/IL-35 p35

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Porcine peripheral blood mononuclear cells treated with LPS, then fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-35 p35

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Porcine IL-12 subunits p35 and p40 share about 85% homology to the human subunits, but differ by containing a 3 aa addition in the p35 subunit and a 4 aa deletion in the p40 subunit. Porcine IL-12 induces proliferation of human lymphoblasts and IFN-gamma secretion by human and porcine lymph nodes. Porcine IL-12 has been detected in lymphoid tissues including inguinal and mesenteric lymph nodes, Peyer's patches, spleen and thymus.

References

Foss, D. et al. (1997) Vet. Immunol. Immunopathol. 57:121.

Long Name

Interleukin 12 p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Alternate Names

CLMF p35; CLMF; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12, subunit p35; IL-12/IL-35 p35; IL-12A; IL35 subunit; IL35p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; p35

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Porcine IL-12/IL-35 p35 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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