ProDots Recombinant Human Noggin Protein

Name: ProDots Recombinant Human Noggin Protein
Brand: R&D Systems
SKU: PRD6057
Price: 647 USD

Catalog #: PRD6057 Datasheet / COA / SDS

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD6057 has been discontinued.

View all Noggin products.

ProDots Recombinant Human Noggin Protein SDS-PAGE

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All Noggin Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

ProDots Recombinant Human Noggin Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).

Source

Mouse myeloma cell line, NS0-derived human Noggin protein
Gln28 - Cys232

Accession #

Q13253

N-terminal Sequence
Analysis

No results obtained: Gln28 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

23 kDa (monomer)

SDS-PAGE

30-33 kDa, reducing conditions

Product Datasheets

PRD6057

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD6057

Reconstitution	For a stock solution, reconstitute at 250 μg/mL in sterile PBS. For immediate use, simply roll ProDot^® directly into cell culture medium.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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1 μg/lane of ProDots^®Recombinant Human Noggin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 30 kDa and 150 kDa, respectively.

Bioactivity

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ProDots^®Recombinant Human Noggin inhibits BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).

Reconstitution Calculator

Background: Noggin

Noggin is a secreted homodimeric antagonist of bone morphogenetic proteins (BMPs). It binds BMP-4 with high affinity and BMP-7 with lower affinity. Noggin regulates BMP activity in neural tube formation, somite patterning, cardiomyocyte development, and skeletal joint formation. Mutations of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. Noggin may also regulate the differentiation of embryonic stem cells into dopaminergic neurons.

Entrez Gene IDs

9241 (Human); 18121 (Mouse)

Alternate Names

NOG; Noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1; SYNS1A