ProDots Recombinant Human R-Spondin 1 Protein

Name: ProDots Recombinant Human R-Spondin 1 Protein
Brand: R&D Systems
SKU: PRD4645
Price: 2154 USD

Catalog #: PRD4645 Datasheet / COA / SDS

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD4645 has been discontinued.

View all R-Spondin 1 products.

ProDots Recombinant Human R-Spondin 1 Protein SDS-PAGE

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All R-Spondin 1 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

ProDots Recombinant Human R-Spondin 1 Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Source

Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Arg31 - Ala263

Accession #

Q2MKA7

N-terminal Sequence
Analysis

Ser21 & Arg31

Predicted Molecular Mass

25.6 kDa

SDS-PAGE

39 kDa, reducing conditions

Product Datasheets

PRD4645

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD4645

Reconstitution	For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll ProDot^® directly into cell culture medium.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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1 μg/lane of ProDots^®Recombinant Human R-Spondin 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 39 kDa.

Bioactivity

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ProDots^®Recombinant Human R-Spondin 1, in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay.

Reconstitution Calculator

Background: R-Spondin 1

R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/beta-catenin signaling regulators. R-Spondin 1 enhances canonical Wnt/beta-catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors. R-Spondin 1 functions in dorsal neural tube development as well as male and female germ cell development. It also induces bone formation, intestinal crypt cell proliferation, angiogenesis, and insulin secretion from pancreatic beta cells.

Long Name

Roof Plate-specific Spondin 1

Entrez Gene IDs

284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)

Alternate Names

Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1