ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein

Name: ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
Brand: R&D Systems
SKU: PRD1845
Price: 566 USD

Catalog #: PRD1845 Datasheet / COA / SDS

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD1845 has been discontinued.

View all Sonic Hedgehog/Shh products.

ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein Bioactivity

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All Sonic Hedgehog/Shh Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

What are ProDots™ Proteins?

ProDots™ proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots™ proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots™ proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots™ proteins are neatly bundled into the shape of a ball. In this form ProDots™ proteins can be rolled directly into cell culture media. ProDots™ proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots™ proteins can be conveniently stored at 4 ºC where they will remain stable for 6 months. The accessible storage and high stability of ProDots™ proteins makes them ideal for quick and easy preparation of cell culture media.

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED₅₀ for this effect is typically 0.1-0.4 μg/mL.

Source

E. coli-derived human Sonic Hedgehog/Shh protein
Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met

Accession #

NP_000184

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

20 kDa

SDS-PAGE

22 kDa, reducing conditions

Product Datasheets

PRD1845

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD1845

Reconstitution	For a stock solution, reconstitute at 100 µg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thawcycles. • 6 months from date of receipt at room temperatureas supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions afterreconstitution.

Scientific Data

Bioactivity

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ProDots^®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is typically 0.1-0.4 μg/mL.

SDS-PAGE

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1 μg/lane of ProDots^®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 22 kDa.

Reconstitution Calculator

Background: Sonic Hedgehog/Shh

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Alternate Names

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS