Protein A Antibody

Catalog # Availability Size / Price Qty
MAB99761-100
MAB99761-SP
Detection of Protein A by Western Blot.
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Protein A Antibody Summary

Specificity
Detects recombinant Protein A in direct ELISA and Western blots.
Source
Monoclonal Rat IgG2A Clone # 946418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant Protein A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
Recombinant Protein A Long and Recombinant Protein A

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Protein A antibody by Western Blot. View Larger

Detection of Protein A by Western Blot. Western blot shows Recombinant Protein A Long, Recombinant Protein A, and Recombinant Protein G (negative control). The left panel of the PVDF membrane was probed with 2 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein A at approximately 33 kDa and Protein A Long at approximately 55 kDa (as indicated). To show specificity, the right panel was probed with Anti-Rat IgG Antibody (negative control). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Protein A by Simple WesternTM. Simple Western lane view recombinant Protein A Long, recombinant Protein A, and recombinant Protein G (negative control), loaded at 0.2 mg/mL. A specific band was detected for Protein A at approximately 40 kDa and Protein A Long at approximately 58 kDa (as indicated) using 20 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein A

The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is used in purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Entrez Gene IDs
3919448 (S. aureus)
Alternate Names
Immunoglobulin G binding protein A; PROA; Protein A; Protein-A; SPA; Staphylococcal protein A

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