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Proteome Profiler Mouse Adipokine Array Kit

Catalog #: ARY013
32 Citations 11 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 38 different obesity-related antibodies
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ARY013
R&D Systems Proteome Profiler Antibody Array Kits
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Product Details
Citations (32)
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Reviews (11)
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Proteome Profiler Mouse Adipokine Array Kit Summary

Kit Summary

To simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary.

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin IGF-I M-CSF
AgRP IGF-II OSM
ANGPT-L3 IGFBP-1 PAI-1/Serpin E1
CCL2/MCP-1/JE IGFBP-2 PTX2
CCL5/RANTES IGFBP-3 PTX3
CRP IGFBP-5 Pref-1
DPPIV/CD26 IGFBP-6 RAGE
Endocan IL-6 RBP4
Fetuin A IL-10 Resistin
FGF acidic IL-11 TIMP-1
FGF-21 Leptin TNF-alpha
HGF LIF VEGF
ICAM-1 Lipocalin-2/NGAL  

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

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Product Datasheet
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Citations for Proteome Profiler Mouse Adipokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Novel role of Quercetin in ameliorating metabolic syndrome via VDR mediated activation of adiponectin/AdipoR2 signaling
    Authors: Sannappa Gowda, NG;Shiragannavar, VD;Karunakara, SH;Veeranna, RP;Suvarna, D;Kumar, DP;Santhekadur, PK;
    Biochemistry and biophysics reports  2024-09-01
  2. Identification of IGF-1 Effects on White Adipose Tissue and Hippocampus in Alzheimer's Disease Mice via Transcriptomic and Cellular Analysis
    Authors: Kim, YK;Jo, D;Arjunan, A;Ryu, Y;Lim, YH;Choi, SY;Kim, HK;Song, J;
    International journal of molecular sciences  2024-02-22
  3. Adipocyte p53 coordinates the response to intermittent fasting by regulating adipose tissue immune cell landscape
    Authors: Reinisch, I;Michenthaler, H;Sulaj, A;Moyschewitz, E;Krstic, J;Galhuber, M;Xu, R;Riahi, Z;Wang, T;Vujic, N;Amor, M;Zenezini Chiozzi, R;Wabitsch, M;Kolb, D;Georgiadi, A;Glawitsch, L;Heitzer, E;Schulz, TJ;Schupp, M;Sun, W;Dong, H;Ghosh, A;Hoffmann, A;Kratky, D;Hinte, LC;von Meyenn, F;Heck, AJR;Blüher, M;Herzig, S;Wolfrum, C;Prokesch, A;
    Nature communications  2024-02-15
  4. High-fat diet induces C-reactive protein secretion, promoting lung adenocarcinoma via immune microenvironment modulation
    Authors: Hsu, WL;Hsieh, YT;Chen, WM;Chien, MH;Luo, WJ;Chang, JH;Devlin, K;Su, KY;
    Disease models & mechanisms  2023-11-01
  5. Oncogenic KRAS drives lipo-fibrogenesis to promote angiogenesis and colon cancer progression
    Authors: Hsu, WH;LaBella, KA;Lin, Y;Xu, P;Lee, R;Hsieh, CE;Yang, L;Zhou, A;Blecher, JM;Wu, CJ;Lin, K;Shang, X;Jiang, S;Spring, DJ;Xia, Y;Chen, P;Shen, JP;Kopetz, S;DePinho, RA;
    Cancer discovery  2023-09-28
  6. Extracellular vesicles are carriers of adiponectin with insulin-sensitizing and anti-inflammatory properties
    Authors: Blandin, A;Amosse, J;Froger, J;Hilairet, G;Durcin, M;Fizanne, L;Ghesquière, V;Prieur, X;Chaigneau, J;Vergori, L;Dray, C;Pradère, JP;Blandin, S;Dupont, J;Ducluzeau, PH;Dubois, S;Boursier, J;Cariou, B;Le Lay, S;
    Cell reports  2023-08-29
  7. Obesity promotes radioresistance through SERPINE1-mediated aggressiveness and DNA repair of triple-negative breast cancer
    Authors: YH Su, YZ Wu, DK Ann, JL Chen, CY Kuo
    Cell Death & Disease, 2023-01-21;14(1):53.  2023-01-21
  8. Fabricating 3-dimensional human brown adipose microtissues for transplantation studies
    Authors: O Wang, L Han, H Lin, M Tian, S Zhang, B Duan, S Chung, C Zhang, X Lian, Y Wang, Y Lei
    Bioactive materials, 2022-10-27;22(0):518-534.  2022-10-27
  9. Nicotinamide riboside kinase 1 protects against diet and age-induced pancreatic beta-cell failure
    Authors: A Cercillieu, J Ratajczak, M Joffraud, JL Sanchez-Ga, G Jacot, A Zollinger, S Métairon, J Giroud-Ger, M Rumpler, E Ciarlo, M Valera-Alb, A Sambeat, C Canto
    Molecular Metabolism, 2022-09-20;0(0):101605.  2022-09-20
  10. Inhibition of the Monocarboxylate Transporter 1 (MCT1) Promotes 3T3-L1 Adipocyte Proliferation and Enhances Insulin Sensitivity
    Authors: T Bailey, A Nieto, P McDonald
    International Journal of Molecular Sciences, 2022-02-08;23(3):.  2022-02-08
  11. Adipose MDM2 regulates systemic insulin sensitivity
    Authors: P Hallenborg, BAH Jensen, E Fjære, RK Petersen, MS Belmaâti, SS Rasmussen, JP Gunnarsson, P Lauritzen, KKY Cheng, M Hermansson, SB Sonne, CS Ejsing, A Xu, I Kratchmaro, M Krüger, L Madsen, K Kristianse, B Blagoev
    Scientific Reports, 2021-11-08;11(1):21839.  2021-11-08
  12. Crosstalk between Drp1 phosphorylation sites during mitochondrial remodeling and their impact on metabolic adaptation
    Authors: M Valera-Alb, M Joffraud, J Miro-Blanc, J Capellades, A Junza, L Dayon, A Núñez Gali, JL Sanchez-Ga, A Valsesia, A Cercillieu, F Söllner, AG Ladurner, O Yanes, C Cantó
    Cell Reports, 2021-08-24;36(8):109565.  2021-08-24
  13. Obesity alters ovarian folliculogenesis through disrupted angiogenesis from increased IL-10 production
    Authors: PK Yang, CH Chou, CC Huang, WF Wen, HF Chen, CT Shun, HN Ho, MJ Chen
    Molecular Metabolism, 2021-02-13;49(0):101189.  2021-02-13
  14. Pineapple Vinegar Regulates Obesity-Related Genes and Alters the Gut Microbiota in High-Fat Diet (HFD) C57BL/6 Obese Mice
    Authors: NE Mohamad, SK Yeap, H Ky, NWC Liew, BK Beh, SY Boo, WY Ho, SA Sharifuddi, K Long, NB Alitheen
    Evid Based Complement Alternat Med, 2020-09-23;2020(0):1257962.  2020-09-23
  15. Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation
    Authors: CL Jiang, WP Jen, CY Tsao, LC Chang, CH Chen, YC Lee
    PLoS Genet., 2020-05-26;16(5):e1008823.  2020-05-26
  16. Chemerin deficiency regulates adipogenesis is depot different through TIMP1
    Authors: CL Huang, LL Xiao, M Xu, J Li, SF Li, CS Zhu, YL Lin, R He, X Li
    Genes & diseases, 2020-04-09;8(5):698-708.  2020-04-09
  17. Functional expression of TRPM7 as a Ca2+ influx pathway in adipocytes
    Authors: H Inoue, M Inazu, M Konishi, U Yokoyama
    Physiol Rep, 2019-10-01;7(20):e14272.  2019-10-01
  18. Immune-Deficient Pfp/Rag2-/- Mice Featured Higher Adipose Tissue Mass and Liver Lipid Accumulation with Growing Age than Wildtype C57BL/6N Mice
    Authors: S Winkler, M Hempel, MJ Hsu, M Gericke, H Kühne, S Brückner, S Erler, R Burkhardt, B Christ
    Cells, 2019-07-25;8(8):.  2019-07-25
  19. Early postnatal soluble FGFR3 therapy prevents the atypical development of obesity in achondroplasia
    Authors: C Saint-Laur, S Garcia, V Sarrazy, K Dumas, F Authier, S Sore, A Tran, P Gual, I Gennero, JP Salles, E Gouze
    PLoS ONE, 2018-04-13;13(4):e0195876.  2018-04-13
  20. Insulin resistance causes inflammation in adipose tissue
    Authors: M Shimobayas, V Albert, B Woelnerhan, IC Frei, D Weissenber, AC Meyer-Gers, N Clement, S Moes, M Colombi, JA Meier, MM Swierczyns, P Jenö, C Beglinger, R Peterli, MN Hall
    J. Clin. Invest., 2018-03-12;0(0):.  2018-03-12
  21. Potential Hypoglycaemic and Antiobesity Effects of Senna italica Leaf Acetone Extract
    Authors: RO Malematja, VP Bagla, I Njanje, V Mbazima, KW Poopedi, L Mampuru, MP Mokgotho
    Evid Based Complement Alternat Med, 2018-02-19;2018(0):5101656.  2018-02-19
  22. Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
    Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
    Cell. Physiol. Biochem., 2017-12-08;44(5):1896-1911.  2017-12-08
  23. Adipose tissue supports normalization of macrophage and liver lipid handling in obesity reversal
    Authors: M Vatarescu, S Bechor, Y Haim, T Pecht, T Tarnovsky, N Slutsky, O Nov, H Shapiro, A Shemesh, A Porgador, N Bashan, A Rudich
    J. Endocrinol., 2017-03-30;0(0):.  2017-03-30
  24. Mfn2 is critical for brown adipose tissue thermogenic function
    Authors: M Boutant, SS Kulkarni, M Joffraud, J Ratajczak, M Valera-Alb, R Combe, A Zorzano, C Cantó
    EMBO J, 2017-03-27;0(0):.  2017-03-27
  25. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017-03-01;0(0):.  2017-03-01
  26. High-fat Diet Enhances Mammary Tumorigenesis and Pulmonary Metastasis and Alters Inflammatory and Angiogenic Profiles in MMTV-PyMT Mice
    Authors: Sneha Sundaram
    Anticancer Res, 2016-12-01;36(12):6279-6287.  2016-12-01
  27. Hsp90 blockers inhibit adipocyte differentiation and fat mass accumulation.
    Authors: Desarzens S, Liao W, Mammi C, Caprio M, Faresse N
    PLoS ONE, 2014-04-04;9(4):e94127.  2014-04-04
  28. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013-03-30;62(2):206-9.  2013-03-30
  29. Efficacy and tolerability of a novel herbal formulation for weight management in obese subjects: a randomized double blind placebo controlled clinical study.
    Authors: Sengupta K, Mishra A, Rao M, Sarma K, Krishnaraju A, Trimurtulu G
    Lipids Health Dis, 2012-09-20;11(0):122.  2012-09-20
  30. Palmitate enhances the differentiation of mouse embryonic stem cells towards white adipocyte lineages.
    Authors: Petrighi Polidori G, Lomax M, Docherty K
    Mol Cell Endocrinol, 2012-04-05;361(1):40-50.  2012-04-05
  31. Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells.
    Authors: Price RS, Cavazos DA, De Angel RE, Hursting SD, Degraffenried LA
    Prostate Cancer Prostatic Dis., 2012-02-14;15(2):135-43.  2012-02-14
  32. Age and sex mediated changes in epicardial fat adipokines.
    Authors: Fei J, Cook C, Blough E, Santanam N
    Atherosclerosis, 2010-07-07;212(2):488-94.  2010-07-07

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Reviews for Proteome Profiler Mouse Adipokine Array Kit

Average Rating: 4.9 (Based on 11 Reviews)

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Proteome Profiler Mouse Adipokine Array Kit
By Khuynh Nguyen on 08/15/2023

The kit works very well

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Anonymous on 11/22/2021

The serum should be kept in 4 degree fridge or used immediately

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Anonymous on 02/19/2021

Consistent resuts.

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Holly Hinrichs on 02/17/2021

Phenomenal kit that is easy to use and reliable.

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Austin Faerber on 02/10/2021

Outstanding kit.

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Ivan Luzardo on 06/04/2019

We evaluated the adipokine profile of two different mice's groups: a high fat diet and a control group fed with a pigment. We observed significant changes in several proteins

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Anonymous on 04/30/2019
Proteome Profiler Mouse Adipokine Array Kit
By Ivan Luzardo on 12/11/2018
Proteome Profiler Mouse Adipokine Array Kit
By Anonymous on 11/13/2018

Tested in 3T3-L1 cells, data matches expectation, very good

Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Yu Shi on 10/19/2017
Proteome Profiler Mouse Adipokine Array Kit ARY013
Proteome Profiler Mouse Adipokine Array Kit
By Anonymous on 10/06/2016
Proteome Profiler Mouse Adipokine Array Kit ARY013