RADA
Purity: ≥98%
Biological Activity
RADA is an orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Scientific Data
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Application of RADA. Using FDAAs RADA and HADA (Catalog # 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale: 5 μm.
For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841
Optical Data for RADA
| Emission Color | Orange |
|---|---|
| λabs | 554 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95000 M-1cm-1 |
| Quantum Yield (φ) | 0.69 |
| Application | dSTORM, reSTORM |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls.
Hsu et al.
Chem.Sci., 2017;8:6313 -
Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria.
García-Heredia et al.
Elife, 2018;7:pii: e37243. -
Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae.
Fenton et al.
Nat.Microbiol., 2017;2:17011
Product Datasheets
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Citations for RADA
The citations listed below are publications that use Tocris products. Selected citations for RADA include:
2 Citations: Showing 1 - 2
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Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria.
Authors: Garcia-Heredia Et al.
ELife 2018;7
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Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape.
Authors: Baranowski Et al.
ELife 2018;7
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