Rat ALK-7 Antibody

Catalog #: MAB5771 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5771
MAB5771-SP

Detection of ALK‑7 in PC-12 cells by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat ALK-7 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat ALK-7 in Western blots. In Western blots, no cross-reactivity with recombinant human Activin R1B is observed.

Source

Monoclonal Mouse IgG_2A Clone # 118311

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat ALK‑7
Leu26-Arg108
Accession # NP_620790

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat ALK‑7 Fc Chimera (Catalog # 577-A7)

Flow Cytometry

0.25 µg/10⁶ cells

PC-12 rat adrenal pheochromocytoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ALK‑7 in PC-12 cells by Flow Cytometry PC-12 cells were stained with Mouse Anti-Rat ALK‑7 Monoclonal Antibody (Catalog # MAB5771, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. ALK-7 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. Rat ALK-7 expression is restricted to the adult central nervous system, suggesting a possible role of ALK-7 during postnatal maturation and maintenance of several neuronal subpopulations in the cerebellum.

References

ten Dijke, P. et al. (1993) Oncogene 8:2879.
ten Dijke, P. et al. (1994) Science 264:101.
Ryden, M. et al. (1996) J. Biol. Chem. 271:30603.

Long Name

Activin Receptor-like Kinase 7

Entrez Gene IDs

130399 (Human); 245921 (Rat)

Alternate Names

activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30