Rat ALK-7 Biotinylated Antibody

Catalog #: BAM5771 Datasheet / COA / SDS

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BAM5771

All ALK-7 Antibodies

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Rat ALK-7 Biotinylated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat ALK-7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Activin R1B is observed.

Source

Monoclonal Mouse IgG_2A Clone # 118311

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat ALK‑7
Leu26-Arg108
Accession # NP_620790

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat ALK‑7 Fc Chimera (Catalog # 577-A7)

Flow Cytometry

2.5 µg/10⁶ cells

Rat cortical stem cells

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of rat brain (hypothalamus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. ALK-7 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. Rat ALK-7 expression is restricted to the adult central nervous system, suggesting a possible role of ALK-7 during postnatal maturation and maintenance of several neuronal subpopulations in the cerebellum.

Long Name

Activin Receptor-like Kinase 7

Entrez Gene IDs

130399 (Human); 245921 (Rat)

Alternate Names

activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30