Rat CD19 Antibody

Catalog #: AF7489
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7489
AF7489-SP

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All CD19 Antibodies

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Citations (2)

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Rat CD19 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CD19 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CD19 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line recombinant rat CD19
Arg19-Gly287
Accession # NP_001013255

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat CD19 by Western Blot. Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD19 at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19 (also surface antigen B4 and Leu12) is a 95-110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19-547). Based on mouse, it contains a 269 aa extracellular region (aa 19-287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20-113 and 171-271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19-287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Alternate Names

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Rat CD19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Combined Mek inhibition and Pparg activation Eradicates Muscle Invasive Bladder cancer in a Mouse Model of BBN-induced Carcinogenesis
Authors: Tate, T;Plumber, SA;Al-Ahmadie, H;Chen, X;Choi, W;Lu, C;Viny, A;Batourina, E;Gartensson, K;Alija, B;Molotkov, A;Wiessner, G;McKiernan, J;McConkey, D;Dinney, C;Czerniak, B;Mendelsohn, CL;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Telophase correction refines division orientation in stratified epithelia
Authors: KJ Lough, KM Byrd, CP Descovich, DC Spitzer, AJ Bergman, GM Beaudoin, LF Reichardt, SE Williams
Elife, 2019-12-13;8(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC