Rat CD4 Antibody

Catalog #: MAB65771 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB65771-100
MAB65771-SP

Detection of rat CD4 in Rat Splenocytes by Flow Cytometry.

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Rat CD4 Antibody Summary

Species Reactivity

Rat

Specificity

Recognizes the rat CD4 cell surface antigen expressed by T helper cells, monocytes and macrophages. Clone OX38 competes for binding with clone W3/25.

Source

Monoclonal Mouse IgG_2B Clone # OX38

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

MLR generated rat T cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Rat splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of rat CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # MAB65771) or (B) Mouse IgG2B isotype control antibody (MAB0041), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and anti-rat CD3 Phycoerythrin-conjugated antibody. Staining was performed using our Staining Membrane Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV‑1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

Omri, B. Crisanti, P. Alliot, F. Marty, M. Rutin, J. Levallois, C. Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3):377. doi:10.1093/intimm/6.3.377.
Wan, Y.Y. & Flavell, R.A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1):20-36. doi:10.1093/jmcb/mjp001.
Wu, H. Myszka, D. G. Tendian, S.W. Brouillette, C.G. Sweet, R.W. Chaiken, I.M. & Hendrickson, W.A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26):15030. doi:10.1073/pnas.93.26.15030.
Doyle, C. & Strominger, J.L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330:256. doi:10.1038/330256a0.
Vignali, D.A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11):5933-5934. doi:10.4049/jimmunol.1090037.
Tasher, D. & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5:67-80. doi:10.2147/tacg.s18693.
Arthos, J. Deen, K.C. Chaikin, M.A. Fornwald, J.A. Sathe, G. Sattentau, Q.J. Sweet, R.W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3):469. doi:10.1016/0092-8674(89)90922-7.
Buttini, M. Westland, C.E. Masliah, E. Yafeh, A.M. Wyss-Coray, T. Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4):441. doi:10.1038/nm0498-441.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4