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Rat CXCR3 PE-conjugated Antibody

Catalog #: FAB8109P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB8109P
Detection of CXCR3 in Rat Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCR3 PE-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CXCR3 in direct ELISAs. Stains rat CXCR3 transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 868013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with rat CXCR3
Accession # Q9J119
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR3 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CXCR3 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with anti-rat CD4 APC-conjugated antibody and either (A) Mouse Anti-Rat CXCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB8109P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR3

CXCR3, also known as CD183, is an approximately 41 kDa (predicted) 7-transmembrane spanning receptor for the chemokines CXCL9, CXCL10, and CXCL11. It is expressed on activated T cells, B cells, and NK cells during inflammation. It is additionally upregulated on solid tumor cells, tumor endothelium, and cancer stem cells. CXCR3 plays a role in leukocyte recruitment to sites of inflammation. It also contributes to Th1 biased responses during autoimune diseases rheumatoid arthritis, systemic erythematosis, and type 1 diabetes. Rat CXCR3 shares 86% and 96% amino acid sequence identity with human and mouse CXCR3, respectively.

Entrez Gene IDs
2833 (Human); 12766 (Mouse); 84475 (Rat)
Alternate Names
CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR

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Citations for Rat CXCR3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ursolic acid inhibits Th17 cell differentiation via STAT3/ROR gamma t pathway and suppresses Schwann cell-mediated Th17 cell migration by reducing CXCL9/10 expression
    Authors: Hua Xu, Ai-ling Yu, Da-peng Zhao, Guang-yuan Meng, Ling Wang, Min Shan et al.
    Innate Immunity
  2. Buyang Huanwu Decoction Attenuates Infiltration of Natural Killer Cells and Protects Against Ischemic Brain Injury
    Authors: B Dou, W Zhou, S Li, L Wang, X Wu, Y Li, H Guan, C Wang, S Zhu, Z Ke, C Huang, Z Wang
    Cell. Physiol. Biochem., 2018-10-24;50(4):1286-1300.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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