Rat IgG1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC005P
Detection of Rat IgG Isotype Control by Flow Cytometry
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Rat IgG1 PE-conjugated Antibody Summary

Specificity
Selected for use as rat IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG1 Clone # 43414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Rat IgG Control by Flow Cytometry RPMI 8226 human multiple myeloma cell line was stained with Rat Anti-Human Syndecan-1/CD138 PE-conjugated Monoclonal Antibody (Catalog # FAB2780P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

Product Datasheets

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Citations for Rat IgG1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Engineered Biomimetic Fibrillar Fibronectin Matrices Regulate Cell Adhesion Initiation, Migration, and Proliferation via alpha 5 beta 1 Integrin and Syndecan‐4 Crosstalk
    Authors: Seungkuk Ahn, Upnishad Sharma, Krishna Chaitanya Kasuba, Nico Strohmeyer, Daniel J. Müller
    Advanced Science
  2. Chronic exposure to biomass ambient particulate matter triggers alveolar macrophage polarization and activation in the rat lung
    Authors: S Wang, Y Chen, W Hong, B Li, Y Zhou, P Ran
    Journal of Cellular and Molecular Medicine, 2022-01-06;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Isotype Control
  3. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018-01-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Rat IgG1 PE-conjugated Antibody

Average Rating: 4 (Based on 1 Review)

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Rat IgG1 PE-conjugated Antibody
By Lu Bai on 07/26/2022
Application: Flow Sample Tested: bone marrow Species: Mouse