Rat IL-1 alpha /IL-1F1 Antibody

Catalog #: AF500
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF500
AF500-SP

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Rat IL‑1 alpha /IL‑1F1 Antibody.

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Citations (3)

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Rat IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL‑1 alpha /IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant mouse IL‑1 alpha /IL‑1F1 and recombinant cotton rat IL‑1 alpha /IL‑1F1 is observed, and less than 15% cross-reactivity with recombinant human IL‑1 alpha /IL‑1F1 and recombinant porcine IL‑1 alpha /IL‑1F1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat IL‑1 alpha /IL-1F1
Ser115-Ser270
Accession # P16598

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat IL‑1 alpha /IL‑1F1 (Catalog # 500-RL)

Neutralization

Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.003-0.009 µg/mL in the presence of 50 pg/mL Recombinant Rat IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Rat IL‑1 alpha /IL‑1F1 Antibody. Recombinant Rat IL-1a/IL-1F1 (Catalog # 500-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500). The ND₅₀ is typically 0.003-0.009 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid (aa) sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporally upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts, and glia (oligodendroglia, astrocytes, and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the aa sequence of mature IL-1 alpha is conserved 60‑70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

References

Dower, S.K. and J.Z. Sims, (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A., ed., Oxford University Press, New York p. 17.

Long Name

Interleukin 1 alpha

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Alternate Names

BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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Citations for Rat IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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