Rat IL-1Rrp2/IL-1R6 Biotinylated Antibody

Catalog #: BAF573 Datasheet / COA / SDS

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BAF573

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Rat IL-1Rrp2/IL-1R6 Biotinylated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL‑1 Rrp2/IL‑1 R6 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse (rm) IL‑18 R is observed, 10% cross‑reactivity with recombinant human (rh) IL‑1 Rrp2 is observed, and less than 5% cross-reactivity with rmIL-1 RI and rmIL-1 RII is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat IL‑1 Rrp2/IL‑1 R6
Gly22-Tyr340
Accession # Q62929

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat IL‑1 Rrp2/IL‑1 R6 Fc Chimera (Catalog # 573-RP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1Rrp2/IL-1R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL‑18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll‑like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Rat IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 319 aa extracellular domain. It shares 84% and 67% amino acid sequence identity with mouse and human IL‑1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL‑1 Rrp2 has been shown to mediate the activation of the transcription factor NF‑kB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1e), with IL‑1 RAcP as a co-factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1d), an IL-1 family ligand that is most closely related to IL‑1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

Long Name

Interleukin 1 Receptor-like 2

Entrez Gene IDs

8808 (Human); 107527 (Mouse); 171106 (Rat)

Alternate Names

IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2; IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2; interleukin 1 receptor-like 2; interleukin-1 receptor-like 2