Rat mGluR2/3 Antibody

Catalog #: PPS078
1 Citation Datasheet / COA / SDS

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PPS078

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All mGluR2/3 Antibodies

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Citations (1)

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Rat mGluR2/3 Antibody Summary

Species Reactivity

Rat

Specificity

Rat ~110 kDa mGluR2 and mGluR3 monomers and ~220 kDa homodimers

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Peptide from the C-terminus region of rat mGluR2 and mGluR3

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of mGluR2/3 by Western Blot Western blot of rat hippocampal lysate showing the specific immunolabeling of the ~110 kDa monomer and the ~220 kDa dimer of mGluR2 and mGluR3.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: mGluR2/3

Rat metabotropic glutamate receptor 2 (mGluR2; also mGlu2) is a 95 kDa, 854 amino acid (aa), 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. Group II receptors include mGluR2 and mGluR3. Both are presynaptic, considered to negatively regulate the release of glutamate, associate with Gi/Go proteins, and inhibit adenylate cyclase, thus decreasing cAMP concentration. The effects of mGluR2 and 3 are not always identical. On microglia, mGluR2 but not mGluR3 stimulation induces TNF-alpha release. mGluR2 is believed to exist as a dimer. Dimerization presumably creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. The cytoplasmic tails of rat mGluR2 and mGluR3 are 64% aa identical; over the last 18 aa, they are 95% aa identical.

References

Rudd, M.T. and J.A. Mccauley (2005) Curr. Top. Med. Chem. 5:869.
Gasparini, F. et al. (2002) Curr. Opin. Pharmacol. 2:43.
Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
Shen, K-Z. and S.W. Johnson (2003) J. Physiol. 533:489.
Taylor, D.L. et al. (2005) J. Neurosci. 25:2952.

Long Name

Metabotropic Glutamate Receptor 2 and 3

Alternate Names

GLUR2; glutamate metabotropic receptor 2; glutamate receptor, metabotropic 2; GPRC1Bmetabotropic glutamate receptor 2; mGlu2; mGluR2; mGluR2/3; MGLUR2glutamate receptor homolog

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Rat mGluR2/3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

mGluR5 in the nucleus accumbens is critical for promoting resilience to chronic stress.
Authors: Shin S, Kwon O, Kang J, Kwon S, Oh S, Choi J, Kim C, Kim D
Nat Neurosci, 2015-05-25;18(7):1017-24.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot