Rat Nogo-A Antibody

Catalog #: MAB30981 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30981
MAB30981-SP

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

All Nogo-A Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Nogo-A Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Nogo-A in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 391401

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat Nogo-A
Glu2-Val172
Accession # Q9JK11

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Customers also Viewed

Nogo-A antibody in Rat Embryo by Immunohistochemistry (IHC-Fr).

Rat Nogo-A Antibody

Cat # MAB3098

Citations (3)

R&D Systems Recombinant Proteins and Enzymes

Recombinant Rat Nogo-A Fc Chimera (aa 1026-1090) Protein, CF

Cat # 3728-NG

Citations (3)

Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF

Cat # 3098-NG

Citations (1)

Recombinant Rat Nogo-A Fc Chimera Protein, CF

Cat # 2445-NG

Citations (2)

Human HAI-1 Antibody

Cat # MAB1048

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat Nogo-A Fc Chimera (Catalog # 2445-NG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo-A

Nogo-A, also known as Reticulon-4, is the longest of four splice variants of Nogo. It is a CNS myelin-associated neurite outgrowth inhibitor that is highly expressed in oligodendrocytes. Nogo-A is synthesized as an 1163 amino acid protein and lacks a signal peptide. Within conserved C-terminal reticulon homology domain (RHD), two transmembrane domains, which are separated by a 66 amino acid extracellular loop, exist. Both the N-terminal domain and the 66 amino acid domain (Nogo-66) can be detected on the cell surface and show neurite outgrowth inhibitory activity. The amino acid sequence of rat Nogo-A N-terminal domain is 76% identical to that of human Nogo-A. Within the RHD domain, rat Nogo-A shares 99% and 97% amino acid sequence identity with mouse and human Nogo, respectively.

Long Name

Reticulon 4A

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Alternate Names

ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A