Rat PDGF-AA Antibody

Catalog #: AF1055
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1055
AF1055-SP

Cell Proliferation Induced by PDGF‑AA and Neutralization by Rat PDGF‑AA Antibody.

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Citations (2)

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Rat PDGF-AA Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat PDGF‑AA in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat (rr) PDGF-AB is observed, 30% cross-reactivity with recombinant human PDGF-AA is observed, and less than 5% cross-reactivity with rrPDGF-BB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat PDGF‑AA
Ser87-Arg196
Accession # AAB59693

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat PDGF‑AA (Catalog # 1055-AA)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of rat intestine and thymus

Neutralization

Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 25 ng/mL Recombinant Rat PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PDGF‑AA and Neutralization by Rat PDGF‑AA Antibody. Recombinant Rat PDGF-AA (Catalog # 1055-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1055). The ND₅₀ is typically 0.2-0.6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-AA

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB,-BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1‑4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that have five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. Rat PDGF-A chain cDNA encodes a 204 amino acid (aa) residue precursor protein with a 20 aa signal peptide, a 65 aa propeptide that is removed by proteolysis, and a 119 aa mature protein. By alternative splicing, a short form lacking 8 C-terminal aa residues also exists. The long form contains the 8 aa basic insert which promotes intracellular cell retention and association with cell matrix. PDGF-A is expressed in multiple cell types and tissues. Based on PDGF-A knockout studies, PDGF-A appears to be important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles and intestinal villus (1).

References

Betshotz, C. et al. (2001) BioEssays 23:494.
Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Long Name

Platelet-derived Growth Factor AA

Entrez Gene IDs

5154 (Human); 25266 (Rat)

Alternate Names

PDGF1; PDGF-A; PDGFAA; PDGF-AA

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Product Datasheet

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Citations for Rat PDGF-AA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naï¿½ve Schwann Cells in the Sciatic Nerve Injury Model
Authors: M Zhang, MH Jiang, DW Kim, W Ahn, E Chung, Y Son, G Chi
Biomed Res Int, 2017-06-20;2017(0):1252851.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
Authors: Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R
Lab. Invest., 2008-07-28;88(10):1090-100.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot