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Rat Reg3B Antibody

Catalog #: AF1996
5 Citations Datasheet / COA / SDS
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AF1996
AF1996-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Reg3B Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Reg3B in direct ELISAs and Western blots. In direct ELISAs, approxmately 75% cross-reactivity with recombinant mouse (rm) Reg3B, and approximately 20% cross-reactivity with rmReg3G is observed, and less than 2% cross-reactivity with recombinant human (rh) Reg3A, rmReg1, rmReg2, rhReg4, rhReg1A, rmReg3A, and rmReg3D is observed. In Western blots, approximately 40% cross-reactivity with rmReg3B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Reg3B

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3B

Rat Reg3B, also named pancreatitis-associated protein 1, Regenerating islet-derived protein 3-beta and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Rat Reg3B has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Long Name
Regenerating Islet-derived IIIb
Entrez Gene IDs
18489 (Mouse); 24618 (Rat)
Alternate Names
PAP1; Reg3B

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Citations for Rat Reg3B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway
    Authors: Huairong Zhang, Andrea Liliam Gomez Corredor, Julia Messina-Pacheco, Qing Li, George Zogopoulos, Nancy Kaddour et al.
    Communications Biology
  2. Bile acid administration elicits an intestinal antimicrobial program and reduces the bacterial burden in two mouse models of enteric infection
    Authors: S Tremblay, G Romain, M Roux, XL Chen, K Brown, DL Gibson, S Ramanathan, A Menendez
    Infect. Immun, 2017-05-23;0(0):.
  3. Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia
    Authors: J Li, H Shi, H Liu, F Dong, Z Liu, Y Lu, L Chen, L Bao, X Zhang
    J. Neurosci., 2019-11-19;40(2):297-310.
    Species: Human, Rat, Transgenic Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A SILAC-Based Method for Quantitative Proteomic Analysis of Intestinal Organoids
    Sci Rep, 2016-11-30;6(0):38195.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats.
    Authors: van Ampting MT, Rodenburg W, Vink C, Kramer E, Schonewille AJ, Keijer J, van der Meer R, Bovee-Oudenhoven IM
    Dig. Dis. Sci., 2009-12-01;54(0):2588.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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