Rat RTN1-A/NSP Antibody

Catalog #: MAB5996 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5996
MAB5996-SP

All RTN1-A/NSP Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat RTN1-A/NSP Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat RTN1-A/NSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat RTN-A2B, recombinant human (rh) RTN1-A, and rhRTN3-A1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 559706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat RTN1-A/NSP
Met73-Ser502
Accession # Q64548

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of rat brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RTN1-A/NSP

Reticulon-1 (RTN1-A/RTN1) is a 135 kDa multi-pass membrane protein localized to the endoplasmic reticulum of neurons. RTN1-A belongs to no known family of proteins. Rat RTN1-A is 777 amino acids (aa) in length and contains two transmembrane segments and a Reticulon domain (aa 592-759). There are also multiple potential sites for phosphorylation. Alternative splicing produces a second isoform known as RTN1-S, which is missing residues corresponding to aa 1-569 in RTN1-A, and has a 20 aa substitution for residues corresponding to aa 570-589. Rat RTN1-A is 82% aa identical to human RTN1-A

Long Name

Reticulon 1A/Neuroendocrine-specific Protein

Entrez Gene IDs

6252 (Human); 116644 (Rat)

Alternate Names

NSP; NSP-A; RTN1; RTN1A; RTN1-A