Rat TCR  gamma /δ APC-conjugated Antibody

Catalog #: FAB6089A Datasheet / COA / SDS

Discontinued Product

FAB6089A has been discontinued.
View all TCR gamma/delta products.
Detection of TCR  gamma /δ in Rat Splenocytes by Flow Cytometry.
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Rat TCR  gamma /δ APC-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TCR gamma /δ in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # V65
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CD3+ TCR alpha / beta - rat T cell hybridoma line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TCR ?/d antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of TCR gamma /δ in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Anti-Rat CD4 Fluorescein-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat TCR ?/d APC-conjugated Monoclonal Antibody (Catalog # FAB6089A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TCR gamma/delta

TCR gamma /δ is associated with CD3 and expressed on a subset of T cells in the thymus, spleen, lymph node, and intestinal epithelium.

References
  1. Kuhnlein, P. et al. (1994) J. Immunol. 153:979.
Entrez Gene IDs
915 (Human); 12500 (Mouse); 25710 (Rat)
Alternate Names
delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCR gamma/delta; TCRG; TiT3; TRG

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