Rat TrkA Antibody Summary
Ala33-Pro418
Accession # P35739
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Rat TrkA by Western Blot. Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TrkA in Rat Dorsal Root Ganglion. TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Porcine TrkA by Immunohistochemistry Immunohistochemical characterization of ChR2-expressing DRG neurons. A, Immunolabeling of ChR2+ neurons (Venus; green) with either NF200, TrkC, CGRP, TrkA, TRPV1, or IB4 (red) in the L4 DRG contralateral and ipsilateral to PNI on day 14. B, Percentage of colocalization of each marker in ChR2+ DRG neurons [n = 4 rats (two to three slices per rat)]. Size-frequency histogram (inset) illustrating the distribution of the cross-sectional areas of ChR2+ NF200+ DRG neurons in the L4 DRG ipsilateral (371 cells) and contralateral (316 cells) to PNI on day 14. Values represent mean ± SEM. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29468190), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat TrkA by Immunohistochemistry Immunohistochemical characterization of ChR2-expressing DRG neurons. A, Immunolabeling of ChR2+ neurons (Venus; green) with either NF200, TrkC, CGRP, TrkA, TRPV1, or IB4 (red) in the L4 DRG contralateral and ipsilateral to PNI on day 14. B, Percentage of colocalization of each marker in ChR2+ DRG neurons [n = 4 rats (two to three slices per rat)]. Size-frequency histogram (inset) illustrating the distribution of the cross-sectional areas of ChR2+ NF200+ DRG neurons in the L4 DRG ipsilateral (371 cells) and contralateral (316 cells) to PNI on day 14. Values represent mean ± SEM. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29468190), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkA
TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.
- Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
- Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Product Datasheets
Citations for Rat TrkA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cartilage-binding antibodies induce pain through immune complex–mediated activation of neurons
Authors: Alex Bersellini Farinotti, Gustaf Wigerblad, Diana Nascimento, Duygu B. Bas, Carlos Morado Urbina, Kutty Selva Nandakumar et al.
Journal of Experimental Medicine
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Dissociation between the relief of skeletal pain behaviors and skin hypersensitivity in a model of bone cancer pain
Authors: Jean-Marc G. Guedon, Geraldine Longo, Lisa A. Majuta, Michelle L. Thomspon, Michelle N. Fealk, Patrick W. Mantyh
Pain
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Kv7.5 is the primary Kv7 subunit expressed in C-fibers
Authors: Chih H. King, Steven S. Scherer
The Journal of Comparative Neurology
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Breast Cancer-Induced Bone Remodeling, Skeletal Pain, and Sprouting of Sensory Nerve Fibers
Authors: Aaron P. Bloom, Juan M. Jimenez-Andrade, Reid N. Taylor, Gabriela Castañeda-Corral, Magdalena J. Kaczmarska, Katie T. Freeman et al.
The Journal of Pain
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Dynamic expression of transcription factor Brn3b during mouse cranial nerve development
Authors: Szilard Sajgo, Seid Ali, Octavian Popescu, Tudor Constantin Badea
Journal of Comparative Neurology
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The majority of myelinated and unmyelinated sensory nerve fibers that innervate bone express the tropomyosin receptor kinase A
Authors: Gabriela Castañeda-Corral, Juan M. Jimenez-Andrade, Aaron P. Bloom, Reid N. Taylor, William G. Mantyh, Magdalena J. Kaczmarska et al.
Neuroscience
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Mechanisms of nerve growth factor signaling in bone nociceptors and in an animal model of inflammatory bone pain
Authors: Sara Nencini, Mitchell Ringuet, Dong-Hyun Kim, Yu-Jen Chen, Claire Greenhill, Jason J Ivanusic
Mol Pain
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Titin is a nucleolar protein in neurons
Authors: Cameron, B;Torres-Hernandez, L;Montague, VL;Lewis, KA;Smith, H;Fox, J;Guo, X;Kalb, RG;George, L;
Research square
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy
Authors: N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner
Cell Reports, 2023-03-21;42(4):112295.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain
Authors: DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha
Oncogene, 2022-06-02;12(1):9241.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch
Authors: K Kanehisa, K Koga, S Maejima, Y Shiraishi, K Asai, M Shiratori-, MF Xiao, H Sakamoto, PF Worley, M Tsuda
Nature Communications, 2022-05-02;13(1):2367.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons
Authors: S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic
Frontiers in Physiology, 2021-07-15;12(0):644929.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis
Authors: K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato
Journal of pharmacological sciences, 2021-04-07;146(3):125-135.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation
Authors: MA Landy, M Goyal, KM Casey, C Liu, HC Lai
Cell Reports, 2021-03-30;34(13):108913.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
Cell Rep, 2020-11-24;33(8):108425.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates
Authors: L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab
Nat Commun, 2020-08-21;11(1):4175.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain
Authors: M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer
J Neuroinflammation, 2020-06-12;17(1):183.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone
Authors: M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic
Molecular pain, 2020-01-01;16(0):1744806920975.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
A cell fitness selection model for neuronal survival during development
Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
Nat Commun, 2019-09-12;10(1):4137.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity
Authors: X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang
Cell Rep, 2019-06-25;27(13):3799-3807.e3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
Authors: S Wiszniak, Q Schwarz
BMC Neurosci, 2019-04-29;20(1):21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F -
PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis
Authors: L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast
Cell Rep, 2019-03-26;26(13):3484-3492.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis
Authors: H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao
Nat Commun, 2019-01-14;10(1):181.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors
Authors: I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner
Mol Pain, 2018-11-02;14(0):1744806918814.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats
Authors: R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda
eNeuro, 2018-02-15;5(1):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur
Authors: SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh
Neuroscience, 2018-02-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
[EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur
Authors: SR Chartier, SA Mitchell, LA Majuta, P Mantyth
Mol Pain, 2017-11-22;0(0):1744806917745.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
J. Neurosci. Methods, 2017-03-07;282(0):34-42.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
Nat Neurosci, 2014-11-24;18(1):145-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
Authors: Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S
J Neurosci, 2014-01-22;34(4):1494-509.
Species: Rat
Sample Types: Whole Cells
Applications: IHC -
Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
Authors: Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D
BMC Neurosci, 2014-01-01;15(0):1.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
J Biol Chem, 2013-03-07;288(16):11144-54.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons
Authors: NJ Yang, J Isensee, DV Neel, AU Quadros, HB Zhang, J Lauzadis, SM Liu, S Shiers, A Belu, S Palan, S Marlin, J Maignel, A Kennedy-Cu, VS Tong, M Moayeri, P Röderer, A Nitzsche, M Lu, BL Pentelute, O Brüstle, V Tripathi, KA Foster, TJ Price, RJ Collier, SH Leppla, M Puopolo, BP Bean, TM Cunha, T Hucho, IM Chiu
Nature Neuroscience, 2021-12-20;0(0):.
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Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification
Authors: Vladimir Vladimirovich Muzyka, Tudor Constantin Badea
Neural Development
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Acute inflammation sensitizes knee-innervating sensory neurons and decreases mouse digging behavior in a TRPV1-dependent manner
Authors: Sampurna Chakrabarti, Luke A. Pattison, Kaajal Singhal, James R.F. Hockley, Gerard Callejo, Ewan St. John Smith
Neuropharmacology
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Secreted herpes simplex virus-2 glycoprotein G alters thermal pain sensitivity by modifying NGF effects on TRPV1
Authors: Jorge Rubén Cabrera, Abel Viejo-Borbolla, Antonio Alcamí, Francisco Wandosell
Journal of Neuroinflammation
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A Local Source of FGF Initiates Development of the Unmyelinated Lineage of Sensory Neurons
Authors: Saïda Hadjab, Marina C. M. Franck, Yiqiao Wang, Ulrich Sterzenbach, Anil Sharma, Patrik Ernfors et al.
The Journal of Neuroscience
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Effects of cerebrolysin on nerve growth factor system in the aging rat brain
Authors: Mikhail Stepanichev, Mikhail Onufriev, Viktor Aniol, Sofia Freiman, Hemma Brandstaetter, Stefan Winter et al.
Restorative Neurology and Neuroscience
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Sensitization of Cutaneous Primary Afferents in Bone Cancer Revealed by In Vivo Calcium Imaging
Authors: Larissa de Clauser, Ana P. Luiz, Sonia Santana-Varela, John N. Wood, Shafaq Sikandar
Cancers (Basel)
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Nociceptor subtypes are born continuously over DRG development.
Authors: Mark A. Landy, Megan Goyal, Helen C. Lai
Developmental Biology
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Preventive or late administration of anti-NGF therapy attenuates tumor-induced nerve sprouting, neuroma formation, and cancer pain
Authors: Juan Miguel Jimenez-Andrade, Joseph R. Ghilardi, Gabriela Castañeda-Corral, Michael A. Kuskowski, Patrick W. Mantyh
Pain
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Muscle-selective RUNX3 dependence of sensorimotor circuit development
Authors: Yiqiao Wang, Haohao Wu, Pavel Zelenin, Paula Fontanet, Simone Wanderoy, Charles Petitpré et al.
Development
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Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold
Authors: Gioele W. Albisetti, Robert P. Ganley, Francesca Pietrafesa, Karolina Werynska, Marília Magalhaes de Sousa, Rebecca Sipione et al.
Neuron
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E12 mouse DRG neurons grown overnight with 10ng/ml NGF, then fixed in 4% PFA, blocked for 1 h with 5% BSA, and incubated overnight in 1% BSA containing AF1056 (1:300). A fluorophore-conjugated secondary antibody was used to visualise reactivity.