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Rat TSH  beta Antibody

Catalog #: MAB4507
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4507
MAB4507-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TSH  beta Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TSH beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human TSH beta.
Source
Monoclonal Mouse IgG2B Clone # 442506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat TSH beta
Phe21-Gly138
Accession # P04652
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat TSH  beta

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSH beta

TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature rat TSH beta shares 92% and 90% aa sequence identity with mouse and human TSH beta, respectively.

Long Name
Thyroid-stimulating Hormone Subunit beta
Entrez Gene IDs
7252 (Human); 22094 (Mouse); 25653 (Rat)
Alternate Names
thyroid stimulating hormone, beta; thyroid-stimulating hormone subunit beta; thyrotropin beta chain; thyrotropin beta subunit; Thyrotropin beta; thyrotropin subunit beta; TSH beta; TSHB; TSH-B; TSH-BETA

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Citation for Rat TSH  beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Novel insights into di?(2?ethylhexyl)phthalate activation: Implications for the hypothalamus?pituitary?thyroid axis
    Authors: H Wu, W Zhang, Y Zhang, Z Kang, X Miao, X Na
    Molecular Medicine Reports, 2021-03-02;23(4):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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