Rat UNC5H2/UNC5B Antibody

Detection of Rat UNC5H2 by Western Blot. Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Rat UNC5H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for UNC5H2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse UNC5H2/UNC5B by Western Blot Expression of UNC5B-D, DCC, neogenin, and A2b in retinas of normal and OIR mice on P12, P17, and P21. (a) Expression of UNC5B-D, DCC, neogenin, and A2b in normal and OIR mice. (b) Relative grayscale of UNC5B-D, DCC, neogenin, A2b, and GAPDH in normal and OIR groups. The UNC5B level of the OIR group is increased significantly compared with the normal group on P17 and P21 (p < 0.05), while the expressions of UNC5C, UNC5D, neogenin, A2b, and DCC show no significant differences between the normal and OIR mice (p > 0.05). In normal mice, DCC expression is greater on P12 than P17 and P21 (p < 0.05), and neogenin expression increases (p < 0.05) from P12 to P21. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25149138), licensed under a CC-BY license. Not internally tested by R&D Systems.

UNC5H2/UNC5B in Rat Brain. UNC5H2/UNC5B was detected in perfusion fixed paraffin-embedded sections of rat brain using Goat Anti-Rat UNC5H2/UNC5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies.

Detection of Mouse UNC5H2/UNC5B by Western Blot RT-PCR analysis of netrin-1 receptor mRNA in retinas of OIR mice. The expressions of UNC5B, UNC5C, UNC5D, neogenin, A2b, and DCC are all detected in retinas of OIR mice, while UNC5A is not detected. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25149138), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse UNC5H2/UNC5B by Western Blot Western blot analysis of the effects of UNC5B shRNA on UNC5B expression in murine retinas. Representative western blot (a) and relative expression of UNC5B in murine retinas (b). UNC5B expression is significantly decreased in the retinas of OIR mice transfected with UNC5B shRNA, compared with scrambled shRNA on P17 (p < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25149138), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse UNC5H2/UNC5B by Functional UNC5B expression outside the CNS.A) Relative UNC5B mRNA expression in brain, heart, intestine, blood, lung, kidney, liver and spleen in C57Bl/6 mice. B)–E) Flowcytometry of murine leukocytes gated and stained for the leukocyte marker CD45 (PerCP labeled), the macrophage marker F4/80 (APC labeled) and UNC5B (PE labeled). F) Immune fluorescence staining of human PMN was performed using anti-UNC5B and CD45 antibodies as primary and Alexa488-conjugated and CruzFluor™ 594-conjugated as secondary antibodies were used as well as isotype matched control antibodies. (Data are mean ± SEM, n = 4 per condition). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22848430), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse UNC5H2/UNC5B by Functional UNC5B expression outside the CNS.A) Relative UNC5B mRNA expression in brain, heart, intestine, blood, lung, kidney, liver and spleen in C57Bl/6 mice. B)–E) Flowcytometry of murine leukocytes gated and stained for the leukocyte marker CD45 (PerCP labeled), the macrophage marker F4/80 (APC labeled) and UNC5B (PE labeled). F) Immune fluorescence staining of human PMN was performed using anti-UNC5B and CD45 antibodies as primary and Alexa488-conjugated and CruzFluor™ 594-conjugated as secondary antibodies were used as well as isotype matched control antibodies. (Data are mean ± SEM, n = 4 per condition). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22848430), licensed under a CC-BY license. Not internally tested by R&D Systems.