Home / IL-21 / Recombinant Canine IL-21 Protein

Recombinant Canine IL-21 Protein

Catalog #: 5849-ML
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
5849-ML-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5849-ML-025
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Canine IL-21 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤8 ng/mL.
Source
E. coli-derived canine IL-21 protein
His18-Ser146, with an N-terminal Met
Accession #
NP_001003347
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
15.0 kDa
SDS-PAGE
14 kDa, reducing conditions

Product Datasheets

You must select a language.

x

5849-ML (with carrier)

Product Datasheet
COA
SDS

You must select a language.

x

5849-ML/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5849-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5849-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-21

Interleukin-21 (IL-21) is an approximately 14 kDa four-helix-bundle cytokine in the family of cytokines that utilize the common gamma chain ( gamma c) as a receptor subunit. gamma c is also a subunit of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (1). IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells (2‑6). It exerts its biological effects through a heterodimeric receptor complex of gamma c and the IL-21-specific IL-21 R (2, 7). Tfh-derived IL-21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses (4, 8, 9). It is also required for the migration of dendritic cells to draining lymph nodes (10). IL-21 regulates several aspects of T cell function. It co‑stimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization (3, 5, 6, 11, 12). It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells (13, 14). IL‑21 R engagement enhances the cytolytic activity and IFN-gamma production of activated NK cells but limits the expansion of resting NK cells (15). In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation (16). Dysregulation of the IL‑21/IL‑21 R system contributes to the development of multiple immunological disorders (1, 17). The canine IL-21 precursor contains a predicted 17 amino acid (aa) signal sequence and a 129 aa mature chain. Mature canine IL-21 shares 75%, 66%, 67%, and 64% aa sequence identity with mature human, mouse, rabbit, and rat IL-21, respectively.

References
  1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
  2. Parrish-Novak, et al. (2000) Nature 408:57.
  3. Coquet, J.M. et al. (2007) J. Immunol. 178:2827.
  4. Vogelzang, A. et al. (2008) Immunity 29:127.
  5. Korn, T. et al. (2007) Nature 448:484.
  6. Nurieva, R. et al. (2007) Nature 448:480.
  7. Asao, H. et al. (2001) J. Immunol. 167:1.
  8. Zotos, D. et al. (2010) J. Exp. Med. 207:365.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  11. Frohlich, A. et al. (2009) Science 324:1576.
  12. Yi, J.S. et al. (2009) Science 324:1572.
  13. Peluso, I. et al. (2007) J. Immunol. 178:732.
  14. Bucher, C. et al. (2009) Blood 114:5375.
  15. Kasaian, M.T. et al. (2002) Immunity 16:559.
  16. Tamagawa-Mineoka, R. et al. (2011) J. Invest. Dermatol. 131:1513.
  17. Ma, J. et al. (2011) Cytokine 56:133.
Long Name
Interleukin 21
Entrez Gene IDs
59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
Alternate Names
CVID11; IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform; Za11

Citations for Recombinant Canine IL-21 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Safety analysis of ex vivo-expanded canine natural killer cells in a xenogeneic mouse model of graft-versus-host disease
    Authors: CJ Kim, SC Park, SH Lee, YJ Lim, M Yoon, JG Park, YB Baek, KO Cho, JW Hong, DJ Shin, SK Kim
    Journal of leukocyte biology, 2021-04-22;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Canine non-B, non-T NK lymphocytes have a potential antibody-dependent cellular cytotoxicity function against antibody-coated tumor cells
    Authors: Y Kim, SH Lee, CJ Kim, JJ Lee, D Yu, S Ahn, DJ Shin, SK Kim
    BMC Vet. Res., 2019-10-14;15(1):339.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Cell Culture

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Canine IL-21 Protein

There are currently no reviews for this product. Be the first to review Recombinant Canine IL-21 Protein and earn rewards!

Have you used Recombinant Canine IL-21 Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review