Recombinant Canine SCF Protein Summary
Product Specifications
Lys26-Ala190, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2278-SC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2278-SC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: SCF/c-kit Ligand
Canine SCF (stem cell factor; also known as c-kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1-4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are four potential N-linked glycosylation sites, two intrachain disulfide bonds, and four
alpha -helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28-40 kDa in size and reflects both N- and O-linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide-linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of SCF has been identified in other species (1). It is membrane bound but lacks the proteolytic cleavage site found in the long form. Thus, it cannot give rise to a soluble molecule. No such isoform has been reported for canine, but it could be assumed to exist. The ratio of long form to short form varies from tissue to tissue (1). Soluble canine SCF shares 88%, 93%, 86%, 83%, 76%, 76%, 86% and 88% aa sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).
- Broudy, V.C. (1997) Blood 90:1345.
- Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
- Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
- Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
- Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
- Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
- Kapur, R. et al. (1998) Blood 91:879.
Citations for Recombinant Canine SCF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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A canine case of malignant melanoma carrying a KIT c.1725_1733del mutation treated with toceranib: a case report and in vitro analysis
Authors: H Tani, R Miyamoto, S Noguchi, S Kurita, T Nagashima, M Michishita, N Yayoshi, K Tamura, M Bonkobara
Bmc Veterinary Research, 2021-04-07;17(1):147.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Characterization of WWOX expression and function in canine mast cell tumors and malignant mast cell lines
Authors: R Makii, H Cook, D Louke, J Breitbach, R Jennings, C Premananda, EM Green, JM Fenger
Bmc Veterinary Research, 2020-10-31;16(1):415.
Species: Canine
Sample Types: Whole Cells
Applications: Cell Culture -
Anti-KIT Monoclonal Antibody Treatment Enhances the Anti-Tumor Activity of Immune Checkpoint Inhibitors by Reversing Tumor-Induced Immunosuppression
Authors: AJ Garton, S Seibel, L Lopresti-M, L Crew, N Janson, S Mandiyan, ES Trombetta, S Pankratz, TM LaVallee, R Gedrich
Mol. Cancer Ther, 2017-01-30;0(0):.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Treatment of canine leukocyte adhesion deficiency by foamy virus vectors expressing CD18 from a PGK promoter.
Authors: Bauer TR, Olson EM, Huo Y
Gene Ther., 2011-01-13;18(6):553-9.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
A functional comparison of canine and murine bone marrow derived cultured mast cells.
Authors: Lin TY, London CA
Vet. Immunol. Immunopathol., 2006-10-06;114(3):320-34.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay
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