Recombinant Chicken Caronte Fc Chimera Protein, CF

Name: Recombinant Chicken Caronte Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1051-CA
Rating: 5 (1 reviews)

Catalog #: 1051-CA 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1051-CA-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Chicken Caronte Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos. 80 ng per embryo induces 25-50% heart reversals. Measured by its binding ability in a functional ELISA. Immobilized rchCaronte/Fc Chimera at 5 µg/mL (100 µL/well) can bind hBMP-4 with a linear range of 0.8-100 ng/mL.

Source

Mouse myeloma cell line, NS0-derived chicken Caronte protein

Chicken Caronte (Asp16 - Asn272) and (Glu18 - Asn272) Accession # NP_990154	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

NP_990154

N-terminal Sequence
Analysis

Asp16 & Glu18

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

56 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Product Datasheets

1051-CA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1051-CA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Caronte

Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.

References