Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 His Protein, CF

Catalog #: 10439-4L Datasheet / COA / SDS

E. coli expressed

Catalog #	Availability	Size / Price	Qty
10439-4L-100

Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag Protein Bioactivity.

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All 4-1BB Ligand/TNFSF9 Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 His Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 9324-4B) is immobilized at 0.1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag (Catalog # 10439-4L) binds with an ED₅₀ of 0.4-2.8 ng/mL.

Source

E. coli-derived cynomolgus monkey 4-1BB Ligand/TNFSF9 protein
Arg68-Glu251, with an N-terminal 6-His tag

Accession #

XP_015296398.1

N-terminal Sequence
Analysis

Met-His6

Predicted Molecular Mass

20 kDa

SDS-PAGE

19-23 kDa, under reducing conditions

Product Datasheets

10439-4L

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10439-4L

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and TCEP with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Cynomolgus Monkey 4-1BB/TNFRSF9/CD137 Fc Chimera (9324-4B) is immobilized at 0.1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag (Catalog # 10439-4L) binds with an ED₅₀ of 0.4-2.8 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag Protein (Catalog # 10439-4L) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-23 kDa.

Reconstitution Calculator

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, cynomolgus Monkey 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10). 4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

References

Wang, C. et al. (2009) Immunol. Rev. 229:192.
Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
Langstein, J. et al. (1998) J. Immunol. 160:2488.
Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
Baessler, T. et al. (2010) Blood 115:3058.
Gullo, C. et al. (2010) PLoS One 5:e10845.
Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Wen, T. et al. (2002) J. Immunol. 168:4897.
Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Entrez Gene IDs

8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)

Alternate Names

41BB Ligand; 4-1BB Ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9