Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein, CF

Catalog #: 10186-RI Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10186-RI-050

Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein Inhibition Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-10-dependent proliferation of MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED₅₀ for this effect is 2-12 ng/mL in the presence of 2 ng/mL Recombinant Human IL‑10 (Catalog # 217-IL).

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-10 R alpha protein

Cynomolgus Monkey IL-10 R alpha (His22-Asn235) Accession # XP_005579838.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005579838.1

N-terminal Sequence
Analysis

His22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa

SDS-PAGE

70-80 kDa, under reducing conditions

Product Datasheets

10186-RI

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10186-RI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Inhibition Activity

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Recombinant Cynomolgus Monkey IL‑10 R alpha Fc Chimera (Catalog # 10186-RI) inhibits IL-10-dependent proliferation of MC/9‑2 mouse mast cells. The ED₅₀ for this effect is 2-12 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey IL-10 R alpha Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 70-80 kDa and 140-160 kDa, respectively.

Reconstitution Calculator

Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 51 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL‑26, IL-28, and IL-29 (1). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature IL-10 R alpha consists of a extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain (12). Within the ECD, cynomolgus monkey IL-10 R alpha shares 95%, 59% and 60% amino acid identity with Human, mouse, and rat IL-10 R alpha, respectively.

References

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
Fernandez, S. et al. (2004) J. Immunol. 172:2613.
Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
Liu, Y. et al. (1994) J. Immunol. 152:1821.

Long Name

Interleukin 10 Receptor alpha

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Alternate Names

CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha