Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein, CF

Catalog #: 11432-CD Datasheet / COA / SDS

Fc Chimera

Catalog #	Availability	Size / Price	Qty
11432-CD-050

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera (Catalog # 11432-CD) binds to recombinant Human CD2 Fc Chimera Protein (Catalog # 1856-CD) with a ED₅₀ of 0.150-2.00 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD58/LFA-3 protein

Cynomolgus Monkey CD58 (Ile29-Arg215) Accession # XP_005542263.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_0055542263.1

N-terminal Sequence
Analysis

Ile29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48 kDa

SDS-PAGE

70-78 kDa, under reducing conditions.

Product Datasheets

11432-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11432-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Chimera Protein (Catalog # 11432-CD) binds to recombinant Human CD2 Fc Chimera Protein (1856-CD) with a ED₅₀ of 0.150‑2.00 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera Protein (Catalog # 11432-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa and 140-160 kDa, respectively.

Reconstitution Calculator

Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a "co-stimulatory" signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4). Blockade of CD58-CD2 interaction on NK cells resulted in diminished production of IFN-gamma and TNF-alpha in response to Human cytomegalovirus (HCMV)-infected cells (7). Expression of CD58 was significantly increased in anisomycin-treated hepatocellular carcinoma (HCC), and may be critical player in NK-mediated immunotherapeutic effects (8).

References

Davis, S.J. and van der Merwe P.A. (1996) Immunol. Today 17:177.
Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
Bolhuis, R.L. et al. (1986) J. Immunol. 136:3939.
Crawford, K. et al. (2003) Blood 102:1745.
Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
Bullens, D.M. et al. (2001) International Immunol. 13:181.
Rölle A. et al. (2016) Eur J Immunol. 46:2420
Kim M. et al. (2018) Sci Rep. 8:10668.

Entrez Gene IDs

965 (Human); 102123839 (Cynomolgus Monkey); 710732 (Rhesus Macaque)

Alternate Names

Ag3; CD58 antigen; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; CD58; FLJ23181; FLJ43722; LFA3; LFA-3; LFA3ag3; lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3