Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera, CF

Catalog #: 9428-GR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9428-GR-100

Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of biotinylated recombinant human GITR Ligand/TNFSF18 that produces 50% of the optimal binding response is 8-40 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey GITR/TNFRSF18 protein

Cynomolgus Monkey GITR/TNFRSF18 (Gln20-Glu155) Accession # XP_005545180	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005545180

N-terminal Sequence
Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Arg21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

49-60 kDa, reducing conditions

Product Datasheets

9428-GR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9428-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera (Catalog # 9428‑GR) is immobilized at 0.5 µg/mL, biotinylated recombinant human GITR Ligand/TNFSF18 binds with an ED₅₀ of 8-40 ng/mL.

Reconstitution Calculator

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature human GITR consists of a 137 amino acid (aa) extracellular domain (ECD) with three tandem TNF R cysteine-rich repeats, a 21 aa transmembrane segment, and a 58 aa cytoplasmic domain (3, 4). Within the ECD region, cyno GITR shares 89.8% and 53.2% aa sequence identity with human and mouse GITR, respectively. Alternative splicing generates an isoform with a short deletion in the cytoplasmic domain and a potentially secreted isoform that is substituted within the third TNF R repeat and lacks the transmembrane and cytoplasmic regions. GITR is expressed on CD4⁺CD25⁺ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (4-6), and it is up-regulated on antigen-activated conventional CD4⁺ and CD8⁺ T cells (3, 4, 6, 7). GITR binding by GITR Ligand/TNFSF18 co-stimulates the proliferation and activation of CD4⁺ or CD8⁺ conventional T cells (3, 7-9). It also induces the proliferation of Treg (8, 10) but inhibits the ability of Treg to suppress immune responses (5, 8, 11-13). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 11), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (10, 14). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (15).

References

Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
Ko, K. et al. (2005) J. Exp. Med. 202:885.
Ji, H. et al. (2004) J. Immunol. 172:5823.
Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Entrez Gene IDs

8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)

Alternate Names

Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18