Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein, CF

Catalog #: 11122-MK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11122-MK-050

Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In the presence of Recombinant Human Agrin (Catalog # 6624-AG), immobilized Recombinant Cynomolgus Monkey MuSK Fc Chimera (Catalog # 11122-MK) at 0.5 µg/mL (100 µL/well) binds Recombinant Human LRP-4 (Catalog # 5948-LR) with an ED₅₀ of 10.0-80.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey MuSK protein

Cyno MuSK (Leu24-Thr495) Accession # XP_005581150.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Leu24

Structure / Form

Disulfide-linked homodimer.

Biotinylated via Avi-tag.

Predicted Molecular Mass

78 kDa

SDS-PAGE

85-105 kDa, under reducing conditions.

Product Datasheets

11122-MK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11122-MK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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In the presence of Recombinant Human Agrin (6624-AG), immobilized Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein (Catalog # 11122-MK) at 0.5 µg/mL (100 µL/well) binds Recombinant Human LRP-4 (5948-LR) with an ED₅₀ of 10.0‑80.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein (Catalog # 11122-MK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa and 170-210 kDa, respectively.

Reconstitution Calculator

Background: MuSK

Muscle-specific kinase (MuSK), also known as muscle skeletal receptor tyrosine-protein kinase, is a single-pass transmembrane protein belonging to the protein kinase superfamily (1). Cynomolgus MuSK consists of an extracellular domain (ECD) with 3 immunoglobulin-like domains and a cysteine-rich domain (CRD), a transmembrane domain, and a cytoplasmic domain with a tyrosine kinase domain (2). Within the mature ECD, cynomolgus MuSK shares 99% and 91% amino acid sequence identity with human and mouse MuSK, respectively. Alternative splicing of MuSK results in multiple isoforms which could result in altered properties and functions (3, 4). MuSK is expressed by skeletal muscle cells and excitatory neurons in the central nervous system (CNS) (1, 2). MuSK is essential for neuromuscular synapse formation and activation of the MuSK signaling cascade is critical for proper signaling between motor neurons and skeletal muscle (1-4). Once activated, MuSK stimulates the pathways that facilitate transcription of genes which encode synaptic proteins in muscle, activate retrograde signaling which promotes presynaptic differentiation, and cluster and anchor acetylcholine receptors (AChRs) (1, 5). Low-density lipoprotein receptor-related protein-4 (Lrp4), is the ligand for MuSK, and its binding affinity is potentiated by agrin (3). Mutant mice lacking agrin, MuSK, and Lrp4 fail to form neuromuscular junctions (NMJ) and subsequently died at birth due to respiratory failure (2, 3). In the presence of mutations which impair MuSK kinase activity or downstream signaling from MuSK, synapses become both structurally and functionally defective, leading to congenital myasthenia (6). The autoimmune disease myasthenia gravis is another neuromuscular disease caused by autoantibodies to AChRs, MuSK or Lrp4 (6). Our Avi-tag Biotinylated MuSK features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity

References

Burden, S.J. et al. (2013) CSH Perspectives Bio. 5(5).
Hubbard, S.R. and Gnanasambandan, K. (2013) Biochem Biophys Acta. 1834(10):2166.
Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
Kuehn, R. et al. (2005) Gene 360:83.
Camurdanoglu, B.Z. et al. (2016) Sci. Rep. 6:33583.
Herbst, R. (2020) Neurosci. Lett. (2020) 716:134676.