Recombinant Cynomolgus Monkey PD-L1/B7-H1 Fc Protein, CF
Recombinant Cynomolgus Monkey PD-L1/B7-H1 Fc Protein, CF Summary
Product Specifications
| Cynomolgus Monkey PD-L1 (Phe19-Thr239) Accession # XP_005581836 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9326-B7
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Cynomolgus Monkey PD-L1/B7-H1 Fc Chimera (Catalog # 9326-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 2-10 μg/mL.
Reconstitution Calculator
Background: PD-L1/B7-H1
B7-H1,
also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane
glycoprotein in the B7 family of immune regulatory molecules (1, 2). Mature
cynomolgus B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD)
with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa
cytoplasmic domain. Within the ECD,
cynomolgus B7-H1 shares 92%, 72%, and 72% aa sequence identity with human,
mouse, and rat B7-H1, respectively. In addition, cynomolgus B7-H1 shares 98%,
94%, 94%, 88%, 78%, 98%, 95%, 94%, and 94%
aa sequence identity with rhesus macaque, chimpanzee, sumatran
orangutan, white-tufted-ear marmoset, Garnett's greater bushbaby, olive baboon,
green monkey, western lowland gorilla, and northern white-cheeked gibbon B7-H1,
respectively. B7-H1 is expressed on inflammatory-activated
immune cells including macrophages, T cells, and B cells (3-6), keratinocytes
(7, 8), endothelial and intestinal epithelial cells (8, 10), as well as a
variety of carcinomas and melanoma (10, 11). B7-H1 is a B7 ligand and binds to B7-1/CD80 and PD-1
receptors on T cells (6, 7, 11-14). It suppresses T cell activation and
proliferation (4, 7, 13, 15) and induces the apoptosis of activated T cells (10).
It plays a role in the development of immune tolerance by promoting T cell anergy
(6, 13) and enhancing regulatory T cell development (15). B7-H1 favors the
development of
anti-inflammatory IL-10 and IL-22 producing dendritic cells (4, 9) and inhibits the development of Th17 cells (15). In cancer, B7-H1 provides
resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic
function of Th22 cells (5, 8, 11, 14). B7-H1/PD-1 coinhibitory pathway was exploited
therapeutically resulting in remarkable outcomes with 20-90% response in
various types of cancer (16).
- Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
- Dong, H. et al. (1999) Nat. Med. 5:1365.
- Tamura, H. et al. (2001) Blood 97:1809.
- Chen, L. et al. (2007) J. Immunol. 178:6634.
- Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
- Tsushima, F. et al. (2007) Blood 110:180.
- Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
- Cao, Y. et al. (2010) Cancer Res. 71:1235.
- Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
- Dong, H. et al. (2002) Nat. Med. 8:793.
- Azuma, T. et al. (2008) Blood 111:3635.
- Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
- Park, J.-J. et al. (2010) Blood 116:1291.
- Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
- Herold, M. et al. (2015) J. Immunol. 195:3584.
- Bardhan K. et al. (2016); Front Immunol. 7:550.
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