Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

Name: Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 7914-TM
Rating: 5 (1 reviews)

Catalog #: 7914-TM 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7914-TM-050

Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein Bioactivity

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All TIM-3 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.5-3 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin‑9 (Catalog # 2045-GA) is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey TIM‑3 Chimera that produces 50% of the optimal binding response is 0.2-1.2 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey TIM-3 protein

Cynomolgus Monkey TIM-3 (Ser22-Arg201) Accession # EHH54703	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

EHH54703

N-terminal Sequence
Analysis

Ser22 & Val24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46.3 kDa (monomer)

SDS-PAGE

60-75 kDa, reducing conditions

Product Datasheets

7914-TM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7914-TM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Cynomolgus Monkey TIM-3 (Catalog # 7914-TM) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.5-3 μg/mL.

Reconstitution Calculator

Background: TIM-3

TIM‑3 (T cell immunoglobulin and mucin domain‑3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig‑like V‑type domain and a Ser/Thr‑rich mucin stalk region (1, 2). Mature cynomolgus TIM‑3 consists of a 182 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. Within the ECD, cynomolgus (or crab‑eating macaque) monkey TIM‑3 shares 81%, 57%, and 56% aa sequence identity with human, mouse, and rat TIM‑3, respectively. TIM‑3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (3‑10). Its binding to Galectin‑9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti‑tumor immunity (11). TIM‑3 ligation attenuates CD8⁺ and Th1 cell responses (11‑13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell‑expressed TIM‑3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (3). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (6). Soluble TIM‑3 is also reported to inhibit the response of T cells to both Ag‑induced and concurrent CD3/CD28 stimulation (15). By contrast, TIM‑3 interactions with Galectin‑9 can trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References

Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
Nakayama, M. et al. (2009) Blood 113:3821.
Anderson, A.C. et al. (2007) Science 318:1141.
Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
Chiba, S. et al. (2012) Nat. Immunol. 13:832.
Monney, L. et al. (2002) Nature 415:536.
Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
Ndhlovu, L.C. et al. (2012) Blood 119:3734.
Gleason, M.K. et al. (2012) Blood 119:3064.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
Geng, H. et al. (2006) J. Immunol. 176:1411.

Long Name

T Cell Immunoglobulin Mucin-3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Alternate Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3