Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF

Catalog #: 10287-N1 Datasheet / COA / SDS

Fc Chimera

Catalog #	Availability	Size / Price	Qty
10287-N1-100

Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 His-tag (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED₅₀ of 2.5-20 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived Nectin-1 protein

Cynomolgus Monkey/Rhesus Macaque Nectin-1 (Gln31-Gly349) Accession # XP_005579992.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005579992.1

N-terminal Sequence
Analysis

No results obtained: Gln31 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

62 kDa

SDS-PAGE

87-101 kDa, reducing conditions

Product Datasheets

10287-N1

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10287-N1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Nectin-3 (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED₅₀ of 2.5-20 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 87-100 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Background: Nectin-1

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means "to connect", indicating the role of nectins in Ca²⁺-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). Based on the similarity with human Nectin-1, the 519 amino acid (aa) cynomolgus Nectin-1 may contain a 30 aa signal sequence, a 328 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3,4). The ECD of cynomolgus Nectin-1 shares 98% and 100% aa identity with human and rhesus monkey Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14). In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence (15).

References

Lopez, M. et al. (1995) Gene 155:261.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
Lopez, M. et al. (2001) J. Virol. 75:5684.
Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
Linehan, M. M. et al. (2004) J. Virol. 78:2530.
Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
Togashi, H. et al. (2006) J. Cell Biol. 174:141.
Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Suzuki, K. et al. (2000) Nat. Genet. 25:427.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
Tampakis, A. et al. (2019) Oncology. 96:318.

Long Name

Poliovirus Receptor Related 1

Entrez Gene IDs

5818 (Human); 58235 (Mouse); 102144697 (Cynomolgus Monkey)

Alternate Names

CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12