Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein, CF

Catalog #: 9884-TR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9884-TR-050

Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein Inhibition Activity

2 Images

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 0.6-3.6 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey TNF RI/TNFRSF1A protein

Cynomolgus Monkey TNF RI/TNFRSF1A (Leu30-Thr211) Accession # NP_001306550	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_001306550

N-terminal Sequence
Analysis

Leu30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47 kDa

SDS-PAGE

55-66 kDa, reducing conditions

Product Datasheets

9884-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9884-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Inhibition Activity

View Larger

Recombinant Cynomolgus Monkey TNF RI/TNFRSF1A Fc Chimera (Catalog # 9884-TR) inhibits Recombinant Human TNF-a (Catalog # 210-TA) mediated cytotoxicity in the L-929 mouse fibroblast cells. The ED₅₀ for this effect is 0.6-3.6 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Cynomolgus Monkey TNF RI/TNFRSF1A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Background: TNF RI/TNFRSF1A

TNF receptor 1 is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). TNF RI is a 455 amino acid (aa) protein that contains a signal sequence and ECD with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a transmembrane domain, and a cytoplasmic domain that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of cynomolgus TNF RI shows 97%, 69%, and 68% aa identity with human, mouse and rat TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). TNF RI is essential for proper development of lymph node germinal centers and Peyer's patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta/TNFSF1B (lymphotoxin-alpha) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14-15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).

References

Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
Chan, F.K. et al. (2000) Science 288:2351.
Schall, T.J. et al. (1990) Cell 61:361.
Peschon, J.J. et al. (1998) J. Immunol. 160:943.
Banner, D.W et al. (1993) Cell 73:431.
Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
Legler, D.F. et al. (2003) Immunity 18:655.
Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
Jin, L. et al. (2000) J. Immunol. 165:5153.
Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
McDermott, M.F. et al. (1999) Cell 97:133.

Long Name

Tumor Necrosis Factor Receptor I

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Alternate Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor