Recombinant Drosophila Decapentaplegic (Dpp) Protein
Recombinant Drosophila Decapentaplegic (Dpp) Protein Summary
Product Specifications
Asp457-Arg588 (Gln473His) & (Pro474Ala), with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
159-DP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
159-DP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Decapentaplegic/DPP
Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional orthologue of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks. Similar to other TGF-beta family ligands, Dpp is synthesized as a large proprotein which is proteolytically processed at the dibasic cleavage site to release the carboxy-terminal domain. Biologically active Dpp is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot and the interchain disulfide bond. Cellular responses to Dpp have been shown to be mediated by the ligand-induced formation of heteromeric complexes of the Drosophila type I, Thick Veins (Tkv), and type II, Punt, serine/threonine kinases. The activated receptor complex induces the phosphorylation of the prototypical Smad, Mad, and subsequent translocation of the Mad-Medea complex to the nucleus where they regulate the transcription of target genes. Secreted extracellular Dpp antagonists, including the short-gastrulation (Sog) and twisted gastrulation (TSG), which bind Dpp and regulate its availability, have been identified.
Citations for Recombinant Drosophila Decapentaplegic (Dpp) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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The Integrator complex desensitizes cellular response to TGF-beta/BMP signaling
Authors: S Liu, GH Baeg, Y Yang, FG Goh, H Bao, EJ Wagner, X Yang, Y Cai
Cell Reports, 2023-01-14;42(1):112007.
Species: Drosophila melanogaster
Sample Types: Whole Cells
Applications: Bioassay -
Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components
Authors: SJ Neal, D Dolezal, N Jusi?, F Pignoni
BMC Dev. Biol., 2019-01-22;19(1):1.
Species: Drosophila
Sample Types: Whole Cells
Applications: Bioassay -
Mapping signaling pathway cross-talk in Drosophila cells
Proc Natl Acad Sci USA, 2016-08-15;0(0):.
Species: Drosophila
Sample Types: Whole Cells
Applications: Bioassay -
Synthetic enzyme-substrate tethering obviates the Tolloid-ECM interaction during Drosophila BMP gradient formation.
Authors: Winstanley J, Sawala A, Baldock C, Ashe H
Elife, 2015-02-02;4(0):.
Species: Drosophila
Sample Types: Protein
Applications: Bioassay -
Ter94/VCP is a novel component involved in BMP signaling.
Authors: Zeng Z, de Gorter D, Kowalski M, ten Dijke P, Shimmi O
PLoS ONE, 2014-12-03;9(12):e114475.
Species: Drosophila
Sample Types: Whole Cells
Applications: Bioassay -
BMP signaling is required for the generation of primordial germ cells in an insect.
Authors: Donoughe S, Nakamura T, Ewen-Campen B, Green D, Henderson L, Extavour C
Proc Natl Acad Sci U S A, 2014-03-03;111(11):4133-8.
Species: Insect - Gryllus
Sample Types: Whole Embryo
Applications: In Vivo -
Crossveinless d is a vitellogenin-like lipoprotein that binds BMPs and HSPGs, and is required for normal BMP signaling in the Drosophila wing.
Authors: Chen J, Honeyager SM, Schleede J
Development, 2012-05-09;139(12):2170-6.
Species: Drosophila
Sample Types: Whole Cells
Applications: Bioassay -
Host and pathogen glycosaminoglycan-binding proteins modulate antimicrobial peptide responses in Drosophila melanogaster.
Authors: Wang Z, Flax LA, Kemp MM, Linhardt RJ, Baron MJ
Infect. Immun., 2010-11-15;79(2):606-16.
Species: Drosophila
Sample Types:
Applications: ELISA Developmet -
The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila.
Authors: Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS
Dev. Cell, 2008-06-01;14(6):940-53.
Species: Drosophila
Sample Types: Recombinant Protein
Applications: Binding Assay
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