Recombinant Hamster ACE-2 His-tag Protein, CF

Catalog #: 10578-ZN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10578-ZN-020

Recombinant Hamster ACE-2 His-tag Protein Binding Activity

3 Images

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Hamster ACE-2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Recombinant Hamster ACE-2 His-tag (Catalog # 10578-ZN) binds Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV) in a functional ELISA. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >700 pmol/min/μg, as measured under the described conditions.

Source

Human embryonic kidney cell, HEK293-derived hamster ACE-2 protein
Gln18-Thr740
with a C-terminal 6-His tag

Accession #

XP_005074266.1

N-terminal Sequence
Analysis

Protein identity confirmed by mass spectrometry.

Predicted Molecular Mass

84 kDa

SDS-PAGE

89-109 kDa, under reducing conditions

Product Datasheets

10578-ZN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10578-ZN

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl₂ and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

View Larger

Recombinant Hamster ACE-2 His-tag (Catalog # 10578-ZN) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) in a functional ELISA.

Enzyme Activity

View Larger

Recombinant Hamster ACE-2 His-tag (Catalog # 10578-ZN) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).

SDS-PAGE

View Larger

2 μg/lane of Recombinant Hamster ACE-2 His-tag Protein (Catalog # 10578-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~104 kDa.

Reconstitution Calculator

Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

References

Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
Yan, R. et al. (2020) Science 367:1444.
Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
Kuba, K. et al. (2005) Nature Med. 11:875.
Hoffman, M. et al. (2020) Cell. 181:1.
Wrapp, D. et al. (2020) Science 367:1260.
Imai, Y. et al. (2005) Nature 436:112.
Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Schrom, E. et al. (2017) Mol Therapy Nuc Acid 7:350.
Jia, H. et al. (2016) Shock. 46:239.
Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
Towler, P. et al. (2004) J Biol. Chem. 279:17996.
Crackower, M.A. et al. (2002) Nature 417:822.

Long Name

Angiotensin I Converting Enzyme 2

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Alternate Names

ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15