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Recombinant Human Activin B Protein

Catalog #: 659-AB
19 Citations 2 Reviews Datasheet / COA / SDS
Newer Version Available: 11517-AB
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This will be discontinued soon. 11517-AB will be the replacement

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659-AB has been discontinued and is replaced by 11517-AB.

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Citations (19)
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Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Activin B Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.3‑1.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Activin B protein
Gly293-Ala407
Accession #
Q53T31
N-terminal Sequence
Analysis
Gly293
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.8 kDa (monomer)
SDS-PAGE
12.4 kDa, reducing conditions

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659-AB (with carrier)

Product Datasheet
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659-AB/CF (carrier free)

Discontinued Product

659-AB

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Activin B

Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha  subunit and one of the various beta  subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and  Xenopus beta D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta A - beta A), activin B ( beta B­ - beta B), activin AB ( beta A ‑  beta B), inhibin A ( alpha - beta A) and inhibin B ( alpha - beta B). At present, little is known about the contribution of the other beta  subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha  subunits share approximately 80% identity. Similarly to other TGF-beta  family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low‑affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.

References
  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
Long Name
Inhibin Subunit Beta B
Entrez Gene IDs
3625 (Human); 3625 (Human); 16324 (Mouse); 16324 (Mouse); 25196 (Rat); 25196 (Rat)
Alternate Names
activin AB beta polypeptide; Activin Beta B; Activin beta-B chain; INHBB; inhibin beta B chain; Inhibin Beta B; Inhibin Subunit Beta B; inhibin, beta B (activin AB beta polypeptide); inhibin, beta B; Inhibin, beta-2

Citations for Recombinant Human Activin B Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth
    Authors: KL Reader, S John-McHaf, S Zellhuber-, T Jowett, DG Mottershea, HE Cunliffe, EJ Gold
    Cancers, 2022-12-27;15(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TGFBR3L is an inhibin B co-receptor that regulates female fertility
    Authors: E Brûlé, Y Wang, Y Li, YF Lin, X Zhou, L Ongaro, CAI Alonso, ERS Buddle, AL Schneyer, CH Byeon, CS Hinck, N Mendelev, JP Russell, M Cowan, U Boehm, F Ruf-Zamojs, M Zamojski, CL Andoniadou, SC Sealfon, CA Harrison, KL Walton, AP Hinck, DJ Bernard
    Science Advances, 2021-12-15;7(51):eabl4391.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  3. TMEPAI/PMEPA1 Is a Positive Regulator of Skeletal Muscle Mass
    Authors: A Hagg, S Kharoud, G Goodchild, CA Goodman, JL Chen, RE Thomson, H Qian, P Gregorevic, CA Harrison, KL Walton
    Front Physiol, 2020-11-04;11(0):560225.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Activins as Dual Specificity TGF-&beta Family Molecules: SMAD-Activation via Activin- and BMP-Type 1 Receptors
    Authors: OE Olsen, H Hella, S Elsaadi, C Jacobi, E Martinez-H, T Holien
    Biomolecules, 2020-03-29;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps
    Authors: L Tengroth, J Arebro, O Larsson, C Bachert, SK Georén, LO Cardell
    Sci Rep, 2018-01-24;8(1):1561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis
    Authors: A Kita, A Kasamatsu, D Nakashima, Y Endo-Sakam, S Ishida, T Shimizu, Y Kimura, I Miyamoto, S Yoshimura, M Shiiba, H Tanzawa, K Uzawa
    J Cancer, 2017-07-05;8(11):2033-2041.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Activin A more prominently regulates muscle mass in primates than does GDF8
    Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
    Nat Commun, 2017-04-28;8(0):15153.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  8. IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes
    Authors: Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba
    J. Biol. Chem., 2017-04-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Occludin expression and regulation in small follicles of the layer and broiler breeder hen
    Authors: CS Stephens, PA Johnson
    Gen. Comp. Endocrinol, 2017-02-24;0(0):.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Regulation of hepcidin expression by inflammation-induced activin B
    Sci Rep, 2016-12-06;6(0):38702.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015-02-23;10(2):e0118097.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Overexpression of activin-A and -B in malignant mesothelioma - attenuated Smad3 signaling responses and ERK activation promote cell migration and invasive growth.
    Authors: Tamminen J, Yin M, Ronty M, Sutinen E, Pasternack A, Ritvos O, Myllarniemi M, Koli K
    Exp Cell Res, 2014-12-31;332(1):102-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. An activin receptor IIA ligand trap promotes erythropoiesis resulting in a rapid induction of red blood cells and haemoglobin.
    Authors: Carrancio S, Markovics J, Wong P, Leisten J, Castiglioni P, Groza M, Raymon H, Heise C, Daniel T, Chopra R, Sung V
    Br J Haematol, 2014-03-18;165(6):870-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. SOX9: a stem cell transcriptional regulator of secreted niche signaling factors.
    Authors: Kadaja, Meelis, Keyes, Brice E, Lin, Mingyan, Pasolli, H Amalia, Genander, Maria, Polak, Lisa, Stokes, Nicole, Zheng, Deyou, Fuchs, Elaine
    Genes Dev, 2014-02-15;28(4):328-41.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15.
    Authors: Mottershead D, Ritter L, Gilchrist R
    Mol Hum Reprod, 2011-09-12;18(3):121-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human, Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  17. Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor.
    Authors: Watanabe K, Hamada S, Bianco C, Mancino M, Nagaoka T, Gonzales M, Bailly V, Strizzi L, Salomon DS
    J. Biol. Chem., 2007-10-09;282(49):35772-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases.
    Authors: Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z
    J. Neurochem., 2007-08-06;103(3):962-71.
    Species: Human
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, Western Blot
  19. Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
    Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
    Dev. Biol., 2006-04-27;295(2):473-85.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Human Activin B Protein
By Pablo Hurtado on 10/09/2020
Application: CellProlif
Recombinant Human Activin B Protein
By Anonymous on 04/07/2020
Application: In vitro bioactivity in cell culture
In vitro bioactivity in cell culture Recombinant Human Activin B Protein 659-AB