Recombinant Human Activin RIIB Fc Chimera (Sf 21), CF

Catalog #: 339-RBB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
339-RBB-100

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Activin RIIB Fc Chimera (Sf 21), CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. Approximately 0.02-0.06 µg/mL of rhActivin RIIB/Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Activin RIIB protein

Met	Human Activin RIIB (Ser19 - Thr 134) Accession # CAA54671.1	IEGRMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus				C-terminus

Accession #

CAA54671.1

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Product Datasheets

339-RBB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

339-RBB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Activin RIIB

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

References

Attisano, L. et al. (1996) Mol. and Cell Biol. 16:1066.
Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Long Name

Activin Receptor IIB

Entrez Gene IDs

93 (Human); 11481 (Mouse)

Alternate Names

activin A receptor, type IIB; Activin receptor type IIB; activin receptor type-2B; Activin RIIB; ActivinRIIB; ACTRIIB; ActR-IIB; ACVR2B; EC 2.7.11; EC 2.7.11.30; MGC116908