Recombinant Human ADAM22 Protein, CF

Recombinant Human ADAM22 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: ADAM22

A disintegrin and metalloproteinase 22 (ADAM22), also known as MDC2, is an 85-90 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM22 contains an N-terminal 197 amino acid (aa) cleavable proregion and a 514 aa extracellular domain (ECD). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, a cysteine‑rich region, and one EGF-like domain (2). The cytoplasmic portion contains two phosphorylation motifs which bind to 14-3-3 proteins to mediate cell adhesion (3, 4). Alternative splicing generates multiple isoforms with a variety of insertions, deletions, and substitutions in the cytoplasmic region (2, 5, 6). Within the peptidase-like and disintegrin domains, human ADAM22 shares 96% sequence identity with mouse and rat ADAM22. ADAM22 is expressed on neurons, astrocytes, and Schwann cells in the cerebral cortex, hippocampus, and cerebellum and also on some low-grade gliomas (5, 7-9). It can be up-regulated in endocrine therapy-resistant breast cancer, and it contributes to tumor cell migration (10). It mediates cell adhesion through binding to Integrins alpha 6, alpha 9, and beta 3 (7). ADAM22 is localized to post-synaptic densities (11). It binds the secreted protein LGI1 which functions as a bridge to link ADAM22 with pre-synaptically expressed ADAM23 (9). The interaction of LGI1 with ADAM22 promotes AMPA receptor expression in hippocampal neurons and strengthens excitatory synapses (9). In axonal juxtaparanodes, ADAM22 is a component of the voltage-gated Kv1 potassium channel that responds to LGI1 (12). It also colocalizes with Kv1 channels in basket cell interneuron terminals of the cerebellum (12). ADAM22 additionally binds to Schwann cell derived LGI4 and is required for LGI4 to promote axon myelination (8). Mice lacking ADAM22 expression exhibit hypomyelination and profound ataxia (13).

Related Research Areas

• Metalloproteases and Regulators