Recombinant Human ADAM28 Protein, CF Summary
Product Specifications
Leu72 & Leu191-Ala623, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9325-AD
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 200 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human ADAM28 (Catalog # 9325-AD) induces adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.250-2.50 μg/mL.
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Background: ADAM28
A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 amino acid (aa) extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3). ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9).
- Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
- McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
- Howard, L. et al. (2000) Biochem. J. 348:21.
- Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
- Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
- Blobel, C.P. (1997) Cell 90:589.
- Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
- Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
- Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.
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