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Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

Catalog #: 9059-CL Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9059-CL-050
Recombinant Human AICL/CLEC-2B Fc Chimera Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human AICL/CLEC-2B Fc Chimera is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Galectin-1 (Catalog # 1152-GA) that produces 50% optimal binding response is 0.6-3 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human AICL/CLEC-2B protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human AICL/CLEC-2B
(Lys26-His149) with a Met79Thr substitution
Accession # Q92478
N-terminus C-terminus
Accession #
Q92478
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
48-65 kDa, reducing conditions

Product Datasheets

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9059-CL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9059-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human AICL/CLEC-2B Fc Chimera Protein Bioactivity View Larger

When Recombinant Human AICL/CLEC-2B Fc Chimera (Catalog # 9059-CL) is coated at 2 µg/mL, Recombinant Human Galectin-1 (Catalog # 1152-GA) binds with a typical ED50 of 0.6-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AICL/CLEC-2B

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).

References
  1. Li, Y. et al. (2014) Front. Immunol. 5:123.
  2. Hamann, J. et al. (1997) Immunogenetics 45:295.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Klimosch, S.N. et al. (2013) Blood 122:2380.
Long Name
Activation-Induced C-Type Lectin
Entrez Gene IDs
9976 (Human)
Alternate Names
Activation-induced C-type lectin; AICL; AICLC-type lectin superfamily member 2; CLEC2B; CLEC-2B; CLECSF2; CLECSF2IFN-alpha-2b-inducing-related protein 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 2 (activation-induced); C-type lectin domain family 2 member B; C-type lectin domain family 2, member B; HP10085; IFN-alpha2b-inducing related protein 1; IFNRG1

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