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Recombinant Human ALCAM Fc Chimera Protein, CF

Catalog #: 656-AL
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
656-AL-100
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Citations (8)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ALCAM Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block adhesion of HuT 78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD). The ED50 for this effect is 0.3-1.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ALCAM/CD166 protein
Human ALCAM
(Trp28-Ala526)
Accession # AAB59499		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
AAB59499
N-terminal Sequence
Analysis
Trp28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
83.5 kDa (monomer)
SDS-PAGE
99-115 kDa, reducing conditions

Product Datasheets

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656-AL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

656-AL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC‑1/DM‑GRASP/BEN in the chicken, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 2). Human ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V‑type and three C2‑type Ig‑like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains (1). Human ALCAM ECD shares 93%, 95% and 96% aa sequence identity with mouse/rat, bovine and porcine/equine ALCAM, respectively. A 570 aa isoform lacks aa 503‑515, while a 555 aa form lacks most of the cytoplasmic domain. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (3, 12).

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  3. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
  4. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
  5. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
  6. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
  7. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
  8. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  9. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  10. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  11. King, J.A. et al. (2010) Mol. Cancer 9:266.
  12. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Citations for Recombinant Human ALCAM Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Activated Leukocyte Cell Adhesion Molecule (ALCAM), a Potential 'Seed' and 'Soil' Receptor in the Peritoneal Metastasis of Gastrointestinal Cancers
    Authors: YM Yang, L Ye, F Ruge, Z Fang, K Ji, AJ Sanders, S Jia, C Hao, QP Dou, J Ji, WG Jiang
    International Journal of Molecular Sciences, 2023-01-03;24(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
    Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
    PLoS ONE, 2022-09-15;17(9):e0274667.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Capture
  3. T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab
    Authors: U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair
    PLoS ONE, 2017-07-03;12(7):e0180088.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Activated leukocyte cell adhesion molecule soluble form: a potential biomarker of epithelial ovarian cancer is increased in type II tumors.
    Authors: Carbotti G, Orengo A, Mezzanzanica D, Bagnoli M, Brizzolara A, Emionite L, Puppo A, Centurioni M, Bruzzone M, Marroni P, Rossello A, Canevari S, Ferrini S, Fabbi M
    Int J Cancer, 2012-12-13;132(11):2597-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Relevance of activated leukocyte cell adhesion molecule (ALCAM) in tumor tissue and sera of cervical cancer patients.
    Authors: Ihnen M, Kress K, Kersten JF, Kilic E, Choschzick M, Zander H, Muller V, Mahner S, Janicke F, Woelber L, Milde-Langosch K
    BMC Cancer, 2012-04-04;12(0):140.
    Applications: ELISA (Standard)
  6. Activated leukocyte cell adhesion molecule: a novel biomarker for breast cancer.
    Authors: Kulasingam V, Zheng Y, Soosaipillai A, Leon AE, Gion M, Diamandis EP
    Int. J. Cancer, 2009-07-01;125(1):9-14.
    Applications: ELISA (Standard)
  7. Mitogen-activated protein kinase pathway activation by the CD6 lymphocyte surface receptor.
    Authors: Ibáñez A, Sarrias MR, Farnos M, Gimferrer I, Serra-Pages C, Vives J, Lozano F
    J. Immunol., 2006-07-15;177(2):1152-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells.
    Authors: Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG
    Blood, 2005-12-13;107(8):3212-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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