Recombinant Human alpha-Synuclein Protein, CF

Catalog #: SP-485 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
SP-485-500

Recombinant Human alpha-Synuclein Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human alpha-Synuclein Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human alpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human alpha -Synuclein concentration of 0.5-2.5 μM.

Source

E. coli-derived human alpha-Synuclein protein
Met1 - Ala140

Accession #

P37840.1

Predicted Molecular Mass

14 kDa

Product Datasheets

SP-485

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-485

Formulation	Supplied as a solution in HEPES, NaCl and DTT.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Scientific Data

SDS-PAGE

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0.5 μg and 1 μg per lane of Recombinant Human alpha-Synuclein (Catalog # SP-485) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a single band at 19 kDa.

Reconstitution Calculator

Background: alpha-Synuclein

alpha -Synuclein is a member of a family of small, soluble proteins that also include beta -, and gamma -Synuclein. alpha -Synuclein is predominantly expressed in neurons of the central nervous system, specifically in the presynaptic region of nerve terminals. This protein cycles between a free, partially unfolded form and a helical, membrane-bound form. alpha -Synuclein can aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms are major components of Lewy Bodies and have been linked to the pathogenesis of Parkinson's Disease dementia. alpha -Synuclein aggregates can also be found associated with amyloid plaques in Alzheimer's Disease.

References

Breydo L. et al. (2012) Biochim. Biophys. Acta. 1822: 261.
Chen R.H. et al. (2013) J. Biol. Chem. 288: 7438.
Li X. et al. (2008) Acta. Biochim. Biophys. Sin (Shanghai) 40: 406.
Surguchov A. (2008) Int. Rev. Cell Mol. Biol. 270: 225.
Xia Q. et al. (2008) Front. Biosci. 13: 3850.

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein