Recombinant Human AMIGO3 Fc Chimera Protein, CF

Catalog #: 9508-AM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9508-AM-050

Recombinant Human AMIGO3 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human AMIGO3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human AMIGO3 protein

Human AMIGO3 (Thr20-Pro378) Accession # Q86WK7	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q86WK7

N-terminal Sequence
Analysis

Thr20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

66 kDa

SDS-PAGE

83-103 kDa, reducing conditions

Product Datasheets

9508-AM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9508-AM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human AMIGO3 Fc Chimera (Catalog # 9508‑AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 1-5 μg/mL.

Reconstitution Calculator

Background: AMIGO3

AMIGO3 (Amphoterin-induced protein 3), also called Alivin-3, is a 68 kDa single-pass type I membrane cell adhesion protein. AMIGO3 belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Human AMIGO3 cDNA encodes 504 amino acids (aa) including a 19 aa signal sequence, 264 aa extracelluar domain, 21 aa transmembrane domain and 100 aa cytoplasmic domain. Human AMIGO3 shares 77.2% and 79.1% aa sequence identity with mouse and rat AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 is expressed in brain tissues, also found in liver, kidney and spleen (3). AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (4). Our in-house data showed that AMIGO3 inhibited anti-CD3 induced IL-2 secretion on CD3⁺ cells, suggesting that AMIGO3 may be involved in T cell activation.

References

Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
SwissProt Accession # Q86WK7.
Kuja-Panula J, et al. (2003) J Cell Biol 160:963.
Ahmed Z, et al. (2013) PLoS One. 8:e61878.

Long Name

Amphoterin-induced Gene and ORF 3

Entrez Gene IDs

386724 (Human); 320844 (Mouse)

Alternate Names

adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552