Recombinant Human Annexin A6 Protein, CF

Catalog #: 5186-A6 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5186-A6-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Annexin A6 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rhAnnexin-A6 can bind rhDecorin with an apparent K_D <2 nM.

Source

E. coli-derived human Annexin A6 protein
Ala2-Asp673, with a C-terminal 6-His tag

Accession #

P08133

N-terminal Sequence
Analysis

Ala2

Predicted Molecular Mass

76.5 kDa

SDS-PAGE

70 kDa, reducing conditions

Product Datasheets

5186-A6

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5186-A6

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60 - 70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5 - 7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

References

Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
Crompton, M.R. et al. (1988) EMBO J. 7:21.
Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.
Grewal, T. et al. (2006) Bioessays 28:1211.
Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.
Takagi, H. et al. (2002) J. Cell Sci. 115:3309.
Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.
Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

Entrez Gene IDs

309 (Human); 11749 (Mouse); 79125 (Rat)

Alternate Names

67 kDa calelectrin; Annexin A6; annexin VI (p68); Annexin VI; Annexin-6; ANX6; ANXA6; calcium-binding protein p68; Calelectrin; calphobindin II; calphobindin-II; CBP68; chromobindin-20; CPB-II ; CPB-II; Lipocortin VI; p68; p70; Protein III