Recombinant Human B7-H6 His-tag Avi-tag Protein, CF

Catalog #: AVI9309 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI9309-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human B7-H6 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKp30 Fc Chimera (Catalog # 1849-NK) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human B7‑H6 His-tag Avi-tag (Catalog # AVI9309) that produces 50% of the optimal binding response is 0.10-0.80 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human B7-H6 protein

Human B7-H6 (Asp25-Ser262) Accession # Q68D85.1	6-His tag	Avi-tag
N-terminus		C-terminus

Accession #

Q68D85.1

N-terminal Sequence
Analysis

Asp25

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

29 kDa

SDS-PAGE

50-58 kDa, under reducing conditions.

Product Datasheets

AVI9309

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9309

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human NKp30 Fc Chimera (1849-NK) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human B7‑H6 His-tag Avi-tag (Catalog # AVI9309) that produces 50% of the optimal binding response is 0.10‑0.80 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human B7‑H6 His-tag Avi-tag Protein (Catalog # AVI9309) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-58 kDa.

Reconstitution Calculator

Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins (1 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21aa transmembrane segment, and a 171aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53%-56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7). Our Avi-tag Biotinylated human B7-H6 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References